Extraction, derivatization, and quantification of analytes

1. A method for determining the presence of one or more analytes in a test sample, the method comprising: a) solid phase extracting of the one or more analytes from the test sample, wherein an elution buffer comprising a derivatizing agent is added for derivatizing and eluting the one or more analytes in a single step, and b) detecting the one or more derivatized analytes using liquid chromatography and mass spectrometry.

2. The method according to claim 1 , wherein the one or more analytes is a compound having a primary amine, a secondary amine, or a phenolic hydroxyl group.

3. The method according to claim 2 , wherein the one or more analytes is a monoamine neurotransmitter including catecholamine or one of its derivatives or metabolites, a sex hormone or one of its derivatives or metabolites, a cannabinoid or one of its derivatives or metabolites, a thyroid hormone or one of its derivatives or metabolites, an opiate, opioid or one of its derivatives or metabolites or an arylcyclohexylamine or one of its derivatives or metabolites, an amphetamine or one of its derivatives or metabolites.

4. The method according to claim 1 , wherein the test sample is a biological sample, a soil sample, or a sample of food stuff.

5. The method according to claim 1 , wherein the derivatizing agent is an acyl halide.

6. The method according to claim 5 , wherein the acyl halide is an acyl chloride.

7. The method according to claim 6 , wherein the acyl chloride is a compound of Formula II:

wherein R 1 , R 2 , R 3 , and R 4 are each independently, H, fluorine, chlorine, bromine, iodine, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or cyclic C 1-8 alkoxy.

8. The method according to claim 6 , wherein the acyl chloride is one of the following compounds:

wherein R 1 , R 2 , R 3 , and R 7 are each independently, H, fluorine, chlorine, bromine, iodine, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or cyclic C 1-8 alkoxy, wherein the fused ring is substituted or unsubstituted and is selected from group consisting of a naphthalene, a quinolone, an isoquinoline, a quinzoline, a benzofuran, an indole, a benzimidazole, a 3,4-dihydro-1H-indene, and a 3,4,dihydro-2H-chromene, wherein the fused ring may be substituted by one or more halogen atoms, selected from the group consisting of fluorine, chlorine, bromine, and iodine, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or a cyclic C 1-8 alkoxy.

9. The method according to claim 7 , wherein the acyl chloride is one of the following compounds:

wherein R 1 and R 2 are each independently, H, fluorine, chlorine, bromine, iodine, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or cyclic C 1-8 alkoxy.

10. The method according to claim 9 , wherein the acyl chloride is one of the following compounds:

11. The method according to claim 6 , wherein the acyl chloride is a compound of Formula VII:

wherein R 1 , R 2 , R 3 , R 4 , and R 5 are each independently H, a halogen atom such as a fluorine, or a chlorine, or a bromine, or an iodine, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or cyclic C 1-8 alkoxy.

12. The method according to claim 11 , wherein the acyl chloride is one of the following compounds:

wherein the fused ring system may be an all carbocyclic or a heterocyclic aromatic system, having a 5-7 membered fused ring, or a nonaromatic carbocyclic, or heterocyclic fused ring system such as 3,4-dihydro-1H-indene, or a 3,4,dihydro-2H-chromene, or having a 4-8 membered fused ring, and wherein the fused ring may be substituted with one or more of a halogen atom, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or cyclic C 1-8 alkoxy.

13. The method according to claim 11 , wherein the acyl chloride is the following compound:

14. The method according to claim 6 , wherein the acyl chloride is a compound of Formula VIII:

wherein R 1 , R 2 , R 3 , R 4 , and R 5 are each independently H, a halogen atom, such as, fluorine, or a chlorine, or a bromine, or an iodine, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or cyclic C 1-8 alkoxy.

15. The method according to claim 14 , wherein the acyl chloride is one of the following compounds:

wherein the fused ring system may be an all carbocyclic or a heterocyclic aromatic system, having a 5-7 membered fused ring, or a nonaromatic carbocyclic, or heterocyclic fused ring system such as 3,4-dihydro-1H-indene, or a 3,4,dihydro-2H-chromene, or having a 4-8 membered fused ring, and wherein the fused ring may be substituted with one or more of a halogen atom, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or cyclic C 1-8 alkoxy.

16. The method according to claim 14 , wherein the acyl chloride is the following compound:

17. The method according to claim 1 , wherein the derivatizing agent is a compound of Formula IX:

wherein R 1 , R 2 , R 3 , R 4 , and R 5 are each independently, a hydrogen, a halogen atom, such as fluorine, chlorine, bromine, or iodine, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or cyclic C 1-8 alkoxy.

18. The method according to claim 17 , wherein the derivatizing agent is one of the following compounds:

wherein the fused ring system may be an all carbocyclic or a heterocyclic aromatic system, such as a naphthalene, a quinolone, an isoquinoline, a quinzoline, a benzofuran, an indole, or a benzimidazole, having a 5-7 membered fused ring, or a nonaromatic carbocyclic, or heterocyclic fused ring system such as 3,4-dihydro-1H-indene, 3,4,dihydro-2H-chromene, having a 4-8 membered fused ring, and wherein the fused ring may be substituted by one or more of a halogen atoms, such as fluorine, chlorine, bromine, iodine, a cyano group, an acetylene group, a propylene group, a vinyl group, a linear C 1-8 alkyl, a branched C 1-8 alkyl, a cyclic C 1-8 alkyl, a substituted vinyl group, a linear C 1-8 alkoxy, a branched C 1-8 alkoxy or cyclic C 1-8 alkoxy.

19. The method according to claim 1 , wherein the mass spectrometry comprises a tandem mass spectrometry technique or a LC-MS/MS technique.

20. The method according to claim 1 , wherein the detection of the one or more analytes is qualitative or quantitative.