Method and probe set for detecting cancer
Methods for detecting cancer that include hybridizing a set of chromosomal probes to a biological sample obtained from a patient, and identifying if aneusomic cells are present in a selected subset of cells obtained from the biological sample are described. A set of chromosomal probes and kits for detecting cancer that include sets of chromosomal probes, are also described.
1. A method of screening for cancer in a subject comprising:
(a) obtaining a kit comprising a set of chromosomal probes consisting of a centromeric probe to chromosome 3 labelled with fluorophore that can be visualized with a red or green filter, a centromeric probe to chromosome 7 labelled with fluorophore that can be visualized with a red or green filter, and a centromeric probe to chromosome 17 labelled with fluorophore that can be visualized with an aqua filter; or consisting of a centromeric probe to chromosome 3 labelled with fluorophore that can be visualized with a red or green filter, a centromeric probe to chromosome 7 labelled with fluorophore that can be visualized with a red or green filter, and a centromeric probe to chromosome 17 labelled with fluorophore that can be visualized with an aqua filter, and a locus specific probe to chromosome 9 labelled with fluorophore that can be visualized with a yellow filter; and
(b) detecting the presence or absence of aneusomic cells in a biological sample by hybridizing said set of chromosomal probes of said kit to cells obtained from said biological sample from said subject and examining the hybridization pattern of said set of chromosomal probes;
wherein the presence of aneusomic cells in said biological sample is indicative of cancer in said subject.
2. The method of claim 1 , wherein said biological sample is selected from the group consisting of urine, blood, cerebrospinal fluid, pleural fluid, sputum, peritoneal fluid, bladder washings, oral washings, tissue samples, touch preps, and fine-needle aspirates.
3. The method of claim 1 , wherein said biological sample is urine.
4. The method of claim 1 , wherein said cancer is selected from the group consisting of bladder cancer, lung cancer, breast cancer, ovarian cancer, prostate cancer, colorectal cancer, renal cancer, and leukemia.
5. The method of claim 1 , wherein said cancer is bladder cancer.
6. The method of claim 1 , wherein said chromosomal probes are covalently attached to said fluorophores.
7. The method of claim 1 , wherein said locus specific probe is 9p21.
8. The method of claim 1 , wherein each chromosomal probe is labelled with a different color fluorophore.