Diagnosis of a neurological disease
The present invention relates to a method for diagnosing a disease, comprising the step detecting in a sample an autoantibody binding to the alpha 3 subunit of the human neuronal Na(+)/K(+) ATPase, an isolated and/or recombinant polypeptide comprising the alpha 3 subunit of the human neuronal Na(+)/K(+) ATPase, a use of a membrane-associated human Na(+)/K(+) ATPase or a variant thereof for the diagnosis of a disease, an isolated and/or recombinant polypeptide comprising the alpha 3 subunit of the human neuronal Na(+)/K(+) ATPase or a variant thereof, for use in the treatment of a disease, an autoantibody binding to said polypeptide and a method for isolating such autoantibody, a pharmaceutical composition, medical or diagnostic device or test kit comprising the polypeptide.
1. A kit comprising:
(1) a carrier,
(2) a cell recombinantly expressing a first polypeptide comprising the alpha 3 subunit of the human neuronal Na(+)/K(+) ATPase, wherein the cell is immobilized on the carrier and is not lysed, and
(3) a labeled anti-human-IgG antibody.
2. The kit of claim 1 , wherein the carrier is a nitrocellulose membrane.
3. The kit of claim 1 , wherein the carrier is a glass plate or slide, biochip, microtiter plate, bead, chromatograph column, or membrane.
4. The kit of claim 1 , further comprising a second polypeptide, wherein the second polypeptide is different from the first polypeptide, and is immobilized on the carrier.
5. The kit of claim 4 , wherein the second polypeptide is a negative or positive control for binding between the first polypeptide and an autoantibody against the alpha 3 subunit of human neuronal Na(+)/K(+) ATPase.
6. The kit of claim 1 , wherein the first polypeptide is a fusion protein.