ILT3 polypeptides and uses thereof
This invention provides a method for inhibiting the rejection of transplanted islet cells, comprising administering to the subject a polypeptide comprising all or a portion of the extracellular domain of ILT3, wherein the polypeptide is water soluble. This invention further provides a method of treating diabetes, by inhibiting the rejection of transplanted islet cells through the administration of the polypeptide to the subject.
1. A method of inhibiting the onset of transplant rejection in a subject who has received, or is about to receive, an islet cell transplant, the method comprising the step of administering to the subject a therapeutically effective amount of a soluble polypeptide comprising the extracellular domain of human ILT3.
2. The method of claim 1 , wherein the polypeptide is administered to the subject subcutaneously, intradermally, intravenously, or intraperitoneally.
3. The method of claim 1 , wherein the polypeptide further comprises a Fc portion of an immunoglobulin, wherein the Fc portion of an immunoglobulin comprises a function-enhancing mutation.
4. The method of claim 3 , wherein the Fc portion of an immunoglobulin is a Fc portion of IgG1.
5. The method of claim 4 , wherein the IgG1 is human IgG1.
6. The method of claim 3 , wherein the function-enhancing mutation in the Fc portion inhibits binding of the Fc portion of an immunoglobulin to an Fc receptor.
7. The method of claim 6 , wherein the function-enhancing mutation in the Fc portion comprises an Asn-->G1n point mutation at the N-linked glycosylation site of human IgG1.
8. The method of claim 1 , wherein the polypeptide is administered to the subject concurrently with, or after, islet cell transplantation.
9. The method of claim 1 , wherein the polypeptide is administered to the subject at onset of islet cell transplant rejection.
10. The method of claim 1 , wherein the subject is a mammal.
11. The method of claim 10 , wherein the subject is a human.
12. The method of claim 1 , further comprising administering to the subject an effective amount of one or more additional immunosuppressive agents.