Markers for preeclampsia
View Patent ↗This document provides methods and materials related to determining whether or not a pregnant mammal (e.g., a pregnant human) has preeclampsia. For example, methods and materials related to the use of urinary podocytes to determine whether or not a pregnant human has preeclampsia are provided.
1. A method for avoiding unnecessary treatment of preeclampsia, said method comprising:
(a) contacting a urine sample collected from a pregnant human during gestational week 33.5±5.6, 34.1±4.2, 34.3±3.8, or 39.2±2.2 with an anti-podocin antibody under conditions that allow binding of said anti-podocin antibody to a podocyte or a fragment of said podocyte wherein, when said urine sample contains podocytes or fragments of said podocytes, anti-podocin antibody complexes comprising said anti-podocin antibody and said podocyte or a fragment of said podocyte are formed,
(b) contacting a urine sample collected from said pregnant human during gestational week 33.5±5.6, 34.1±4.2, 34.3±3.8, or 39.2±2.2 with an anti-synaptopodin antibody under conditions that allow binding of said anti-synaptopodin antibody to a podocyte or a fragment of said podocyte wherein, when said urine sample contains podocytes or fragments of said podocytes, anti-synaptopodin antibody complexes comprising said anti-synaptopodin antibody and said podocyte or a fragment of said podocyte are formed,
(c) detecting an absence of an elevated level of said anti-podocin antibody complexes in step (a) as compared to corresponding urine levels from pregnant humans lacking preeclampsia,
(d) detecting an absence of an elevated level of said anti-synaptopodin antibody complexes in step (b) as compared to corresponding urine levels from pregnant humans lacking preeclampsia, and
(e) proceeding with treatment of said pregnant human in a manner that avoids unnecessary treatment of preeclampsia based on the urine levels of complexes detected in step c) and step d) in the pregnant human.