DNA/RNA sequencing using a semiconducting nanopore
The present disclosure provides novel apparatus including, though not necessarily limited to, biosensors utilizing semiconductor materials in electrolyte solutions and methods for using the same. The biosensors rely on a unique property wherein a charged body in the electrolyte solution produces a detectable change in the local conductivity of the semiconductor as the body approaches or travels near the semiconductor.
1. A method for detecting an unlabeled target in an electrolyte solution comprising:
providing at least one detecting unit comprising a doped semiconductor material having a pore and a detector configured to measure the conductivity of the doped semiconductor material surrounding the pore; and
flowing the electrolyte solution comprising the unlabeled target through the pore;
wherein a change in the local conductivity of the doped semiconductor material surrounding the pore as the unlabeled target passes through the pore indicates the presence of the unlabeled target in the electrolyte solution.
2. The method of claim 1 wherein the target is a polymer and changes in local conductivity of the doped semiconductor material surrounding the pore enables the identification of one or more monomer within the polymer.
3. The method of claim 2 wherein the polymer is a nucleic acid sequence and changes in local conductivity of the doped semiconductor material surrounding the pore enables the identification of each nucleotide in the nucleic acid sequence in order.
4. The method of claim 3 wherein the nucleic acid sequence is single-stranded.
5. The method of claim 3 wherein the nucleic acid sequence is double-stranded.
6. The method of claim 2 wherein the polymer is an amino acid sequence and the changes in local conductivity of the doped semiconductor material surrounding the pore enables the identification of each amino acid in the amino acid sequence in order.
7. The method of claim 1 wherein the detecting unit is part of a plurality of detecting units, wherein each detecting unit within the plurality comprises a doped semiconductor material having a pore and wherein all of the pores are in fluid communication with each other.
8. The method of claim 7 wherein at least two of the detecting units are identical in shape and composition.
9. The method of claim 7 wherein at least two of the detecting units differ in either shape, composition, or both.
10. The method of claim 9 wherein at least one of the detecting units comprises a first pore formed within an n-doped semiconductor material and at least one of the other detecting units comprises a second pore formed within an p-doped semiconductor material.
11. A method for detecting a target in an electrolyte solution comprising:
providing at least one detecting unit comprising a doped semiconductor material having a pore and a detector configured to measure the conductivity of the doped semiconductor material surrounding the pore; and
flowing the electrolyte solution and target through the pore;
wherein the passage of the target through the pore changes the local conductivity of the doped semiconductor material surrounding the pore; and
detecting the change in the local conductivity of the doped semiconductor material.
12. The method of claim 11 further comprising obtaining the target and suspending it in electrolyte solution without modifying the target by adding a label.
13. The method of claim 11 further comprising suspending the target in electrolyte solution without amplifying the target.