Method of supplementing cytokine, chemokine and growth factors in donor human milk
Donor milk has become a standard of care for feeding preterm infants, particularly those with gestational ages of 34 weeks or less, whose mothers are not lactating or not producing sufficient milk quantities. However, prior to distribution, donor milk is required to undergo pasteurization, typically using the Holder method, which is believed to destroy immune proteins in the milk and denature many other proteins. Donor milk has been found to contain concentrations of chemokines, cytokines, and growth factors, evidencing the value of donor milk over formula. In light of the findings, donor milk is supplemented with chemokines, cytokines, and growth factors that are found to be lower in the donor milk as compared to mother's own milk.
1. A method of supplementing breast milk, comprising:
obtaining pasteurized breast milk;
determining a level of at least one protein factor in the pasteurized breast milk, wherein the at least one protein factor is a cytokine, chemokine, or growth factor selected from the group consisting of IL-4, MCP-1, MIP-1α, and a combination thereof;
comparing the level of the at least one protein factor in the pasteurized breast milk to levels of the at least one protein factor in preterm milk;
identifying low levels or deficient levels in the at least one protein factor; and
adding at least one additive to the breast milk, wherein the additive is a cytokine, chemokine, or growth factor to remediate low levels or deficient levels in the at least one protein factor.
2. The method of claim 1 , further comprising:
determining the level of at least one supplemental protein factor, wherein the at least one supplemental protein factor is one or more cytokine, chemokine, or growth factors selected from the group consisting of IL-10, IL-6, IP-10, TNF-α, IL-8, and a combination thereof; and
adding the at least one supplemental protein factor to remediate low levels or deficient levels in the at least one supplemental protein factor.
3. The method of claim 2 , wherein the at least one protein factor is IL-4 and the at least one supplemental protein factor is selected from the group consisting of IL-10, IL-8, IP-10, TNF-α, and a combination thereof.
4. The method of claim 3 , wherein the at least one additive is added to bring low levels or deficient levels of the protein factor within the range 9 pg/ml to 18 pg/ml for IL-4, 75 pg/ml to 10400 pg/ml for MCP-1, or 10 pg/ml to 493 pg/ml for MIP1α;
and wherein a supplemental protein factor is added to bring low levels or deficient levels of the supplemental protein factor within the range:
8 pg/ml to 98 pg/ml for IL-10;
17 pg/ml to 117 pg/ml for IL-6;
18 pg/ml to 254 pg/ml for TNF-α; or
a combination thereof.
5. The method of claim 4 , wherein the at least one additive is added to bring low levels or deficient levels of the protein factor to 13 pg/ml for IL-4; and
wherein a supplemental protein factor is added to bring low levels or deficient levels of the supplemental protein factor to a preterm value selected from the group consisting of 11 pg/ml for IL-10, 11.8 pg/ml for IL-10, 12 pg/ml for IL-10, 17.69 pg/ml for IL-6, 26.9 pg/ml for IL-6, 28 pg/ml for IL-6, 21.17 pg/ml for TNF-α, 23.2 pg/ml for TNF-α, 24 pg/ml for TNF-α, and a combination thereof.
6. The method of claim 1 , wherein the at least one additive is added to bring low levels or deficient levels of the at least one protein factor within the range:
9 pg/ml to 151 pg/ml for IL-4, 75 pg/ml to 10400 pg/ml for MCP-1, or 10 pg/ml to 493 pg/ml for MIP1α.
7. The method of claim 6 , wherein the at least one additive is added to bring low levels or deficient levels of the at least one protein factor to a preterm value of 13 pg/ml for IL-4, 13.54 pg/ml for IL-4, or 15.7 pg/ml for IL-4.
8. The method of claim 1 , wherein the pasteurized breast milk was treated at 62.5° C. for 30 minutes, 63° C. for 30 minutes, 62° C. for 5 seconds, 65° C. for 5 seconds, or 72° C. for 5 seconds.
9. The method of claim 8 , wherein the pasteurized breast milk is preterm milk.
10. The method of claim 1 , wherein the pasteurized breast milk is preterm milk.
11. The method of claim 1 , wherein the pasteurized breast milk is human breast milk.
12. The method of claim 1 , further comprising adding at least one lactation protein, wherein the lactation protein is selected from the group consisting of lysozyme, lactoferrin, and a combination thereof;
wherein the level of lysozyme is between about 63 g/L and about 173 g/L; and
wherein the level of lactoferrin is between about 0.78 g/L and about 1.33 g/L.
13. The method of claim 2 , further comprising adding EGF to remediate low levels or deficient levels in EGF.
14. A method of supplementing formula, comprising:
obtaining formula for pre-term infants;
determining the level of at least one protein factor in the formula for pre-term infants;
comparing the level of the at least one protein factor in the formula for pre-term infants to the level of the at least one protein factor in preterm milk; and
adding at least one additive to the formula for pre-term infants, wherein the additive is the at least one protein factor selected from the group consisting of IL-4, MCP-1, MIP-1α, and a combination thereof.
15. The method of claim 14 , further comprising:
determining the level of at least one supplemental protein factor, wherein the at least one supplemental protein factor is one or more cytokine, chemokine, or growth factors selected from the group consisting of IL-10, IL-6, TNF-α, IL-8, and a combination thereof; and
adding the at least one supplemental protein factor to remediate low levels or deficient levels in the at least one supplemental protein factor.
16. The method of claim 15 , wherein the at least one protein factor is IL-4 and the at least one supplemental protein factor is selected from the group consisting of IL-10, IL-8, IP-10, TNF-α, and a combination thereof.
17. The method of claim 16 , wherein the at least one additive is added to bring low levels or deficient levels of the protein factor within the range 9 pg/ml to 18 pg/ml for IL-4, 75 pg/ml to 10400 pg/ml for MCP-1, or 10 pg/ml to 493 pg/ml for MIP1α;
and wherein a supplemental protein factor is added to bring low levels or deficient levels of the supplemental protein factor within the range:
8 pg/ml to 15 pg/ml for IL-10;
17 pg/ml to 31 pg/ml for IL-6;
20 pg/ml to 28 pg/ml for TNF-α; or
a combination thereof.
18. The method of claim 17 , wherein the at least one additive is added to bring low levels or deficient levels of the protein factor to 13 pg/ml for IL-4 and a supplemental protein factor value selected from the group consisting of 12 pg/ml for IL-10, 28 pg/ml for IL-6, 24 pg/ml for TNF-α, and a combination thereof.
19. The method of claim 15 , further comprising adding EGF to remediate low levels or deficient levels in EGF.
20. The method of claim 14 , further comprising adding at least one lactation protein, wherein the lactation protein is selected from the group consisting of lysozyme, lactoferrin, and a combination thereof;
wherein the level of lysozyme is between about 63 g/L and about 173 g/L;
wherein the level of lactoferrin is between about 0.78 g/L and about 1.33 g/L.