IP Library › Granted Patent US 10,582,676
Granted Patent B2
US 10,582,676 · App. 14/742,503 · Granted Mar 10, 2020

Breeding, production, processing and use of specialty cannabis

Inventors: Mark Anthony Lewis (Los Angeles, CA); Michael D Backes (Los Angeles, CA); Matthew W Giese (Los Angeles, CA)
Assignee: Biotech Institute, LLC
A01H5/02A01H1/04A01H4/008A01H5/12A01H6/28
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Quick Facts
Patent No.
US 10,582,676
App. No.
14/742,503
Granted
Mar 10, 2020
Kind
B2
Abstract

The invention provides compositions and methods for the breeding, production, processing and use of specialty cannabis.

Claims (43)

1. A cannabis plant YEL03xTP05, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof, which produces a female inflorescence, said inflorescence comprising:

a) a terpene profile in which myrcene is not the dominant terpene;

b) a terpene oil content greater than 1.0%;

c) a propyl cannabinoid content of at least 1.0%;

d) a B T /B T genotype; and

e) a terpene profile in which beta caryophyllene is the most abundant terpene;

wherein the propyl cannabinoid content is defined as the content of the acidic and decarboxylated variants of tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabichromevarin (CBCV), and cannabigerovarin (CBGV) as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight of the inflorescence; wherein the terpene profile consists of terpinolene, alpha phellandrene, beta ocimene, carene, limonene, gamma terpinene, alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alpha terpineol, alpha humulene, beta caryophyllene, linalool, caryophyllene oxide, and myrcene of a plant, and wherein the terpene oil content is determined by the additive content of the terpenes in the terpene profile; wherein the terpene contents are measured by gas chromatography-flame ionization detection (GC-FID) and calculated based on dry weight of the inflorescence; and wherein cellular cultures of said plant are deposited under Bigelow NCMA Accession No. 201912001.

2. The cannabis plant, or an asexual clone of said cannabis plant, or plant part, tissue, or cell thereof of claim 1 , wherein the female inflorescence comprises a propyl cannabinoid content of at least 3.0%.

3. A method of breeding for a cannabis plant, wherein said cannabis plant produces an inflorescence comprising a terpene oil content greater than 1.0%, a propyl cannabinoid content of at least 1.0%, and a terpene profile in which myrcene is not the dominant terpene, said method comprising:

(i) making a cross between a first cannabis plant of claim 1 , or an asexual clone of said first cannabis plant and a second cannabis plant to produce an F1 seed:

(ii) harvesting the resulting seed;

(iii) growing one or more of said harvested seed; and

(iv) selecting a cannabis plant with a desired phenotype;

wherein the resulting selected cannabis plant produces an inflorescence comprising a terpene oil content greater than 1.0%, a propyl cannabinoid content of at least 1.0%, and a terpene profile in which myrcene is not the dominant terpene.

4. A method of producing a cannabis plant, wherein said cannabis plant produces an inflorescence comprising a terpene oil content greater than 1.0%, a propyl cannabinoid content of at least 1.0%, a terpene profile in which myrcene is not the dominant terpene, a B T /B T genotype, and a terpene profile in which beta caryophyllene is the most abundant terpene, said method comprising:

(i) obtaining a cannabis seed, cutting, or plant cell, from a cannabis plant, or an asexual clone of said cannabis plant of claim 1 ;

(ii) placing said cannabis seed, cutting, or plant cell in an environment conducive to growth; and

(iii) allowing said cannabis seed, cutting, or plant cell to produce a cannabis plant;

wherein the produced cannabis plant produces an inflorescence comprising a terpene oil content greater than 1.0%, a propyl cannabinoid content of at least 1.0%, a terpene profile in which myrcene is not the dominant terpene, a B T /B T genotype, and a terpene profile in which beta caryophyllene is the most abundant terpene.

5. An edible product comprising cannabis tissue from the inflorescence of claim 1 .

6. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein said plant part is selected from the group consisting of: trichomes, sun leaves, or inflorescences.

7. A regenerable cell from a cannabis plant designated YEL03xTP05, wherein cellular cultures of said plant are deposited under Bigelow NCMA Accession No. 201912001.

8. A cannabis plant, or plant part, tissue, or cell thereof produced by regenerating the cell of claim 7 , wherein said cannabis plant, or plant part, tissue, or cell thereof comprises a cannabinoid profile and a terpene profile as set forth in Tables 43 and 44, respectively.

9. The cannabis plant part of claim 8 , wherein said plant part is selected from the group consisting of: stems, trichomes, leaves, and flower buds.

10. A cannabis clone regenerated from the cannabis plant of claim 8 .

11. An edible product comprising the cannabis plant, or plant part, tissue, or cell thereof of claim 8 .

12. A method of producing an F1 cannabis seed, said method comprising crossing the plant of claim 8 with a different cannabis plant, and harvesting the resultant F1 cannabis seed.

13. The F1 hybrid cannabis seed produced by the method of claim 12 .

14. An F1 hybrid cannabis plant produced by growing the F1 hybrid cannabis seed of claim 13 , wherein said F1 hybrid plant produces a female inflorescence, said inflorescence comprising:

a) a terpene profile in which myrcene is not the dominant terpene;

b) a terpene oil content greater than 1.0%;

c) a propyl cannabinoid content of at least 1.0%;

d) a B T /B T genotype; and

e) a terpene profile in which beta caryophyllene is the most abundant terpene;

wherein the propyl cannabinoid content is defined as the content of the acidic and decarboxylated variants of tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabichromevarin (CBCV), and cannabigerovarin (CBGV) as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight of the inflorescence; wherein the terpene profile consists of terpinolene, alpha phellandrene, beta ocimene, carene, limonene, gamma terpinene, alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alpha terpineol, alpha humulene, beta caryophyllene, linalool, caryophyllene oxide, and myrcene of a plant; wherein the terpene oil content is determined by the additive content of the terpenes in the terpene profile; and wherein the terpene contents are measured by gas chromatography-flame ionization detection (GC-FID) and calculated based on dry weight of the inflorescence.

15. A cannabis clone regenerated from the F1 hybrid cannabis plant of claim 14 , wherein said cannabis clone produces a female inflorescence, said inflorescence comprising:

a) a terpene profile in which myrcene is not the dominant terpene;

b) a terpene oil content greater than 1.0%;

c) a propyl cannabinoid content of at least 1.0%;

d) a B T /B T genotype; and

e) a terpene profile in which beta caryophyllene is the most abundant terpene;

wherein the propyl cannabinoid content is defined as the content of the acidic and decarboxylated variants of tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabichromevarin (CBCV), and cannabigerovarin (CBGV) as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight of the inflorescence; wherein the terpene profile consists of terpinolene, alpha phellandrene, beta ocimene, carene, limonene, gamma terpinene, alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alpha terpineol, alpha humulene, beta caryophyllene, linalool, caryophyllene oxide, and myrcene of a plant; wherein the terpene oil content is determined by the additive content of the terpenes in the terpene profile; and wherein the terpene contents are measured by gas chromatography-flame ionization detection (GC-FID) and calculated based on dry weight of the inflorescence.

16. An edible product comprising cannabis tissue from the F1 hybrid cannabis plant of claim 14 .

Assignments (5)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 6, 2025
From: BIOTECH INSTITUTE, LLC
To: OVEST, INC.
Reel/Frame 072809/0984 →
MERGER AND CHANGE OF NAME Recorded Nov 6, 2025
From: OVEST, INC.; TERPENE BELT, INC.
To: TERPENE BELT, INC.
Reel/Frame 072811/0914 →
CHANGE OF NAME Recorded Nov 6, 2025
From: TERPENE BELT, INC.
To: NEXUS AGRISCIENCE, INC.
Reel/Frame 073512/0218 →
SECURITY INTEREST Recorded Jun 24, 2025
From: TERPENE BELT, INC.
To: BRIDGE BANK, A DIVISION OF WESTERN ALLIANCE BANK
Reel/Frame 071507/0943 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 28, 2016
From: LEWIS, MARK ANTHONY; BACKES, MICHAEL D; GIESE, MATTHEW W.
To: BIOTECH INSTITUTE, LLC
Reel/Frame 037611/0392 →
Continuity (4)
Continuation 14216744 · Mar 17, 2014
Provisional Application 61801528 · Mar 15, 2013
Provisional Application 61897074 · Oct 29, 2013
Related Publication 20150366154A1 · Dec 24, 2015