Polypeptides having cellobiohydrolase activity and polynucleotides encoding same

1. A process for degrading a cellulosic material, comprising: treating the cellulosic material with a polypeptide having cellobiohydrolase activity selected from the group consisting of:

(a) apolypeptide having at least 90% sequence identity to amino acids 19 to 464 of SEQ ID NO: 2;

(b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 55 to 1895 of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC. 0.2% SDS at 70° C.;

(c) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; or the cDNA sequence thereof; and

(d) a fragment of the polypeptide of (a), (b), or (c) that has cellobiohydrolase activity.

2. A process for producing a fermentation product, comprising:

(a) saccharifying a cellulosic material with an enzyme composition in the presence of a polypeptide

selected from the group consisting of:

(1) a polypeptide having at least 90% sequence identity to amino acids 19 to 464 of SEQ ID NO: 2;

(2) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 55 to 1895 of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15minutes using 2×SSC. 0.2% SDS at 70° C.;

(3) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; or the cDNA sequence thereof; and

(4) a fragment of the polypeptide of (1), (2), or (3) that has cellobiohydrolase activity; and

(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product.

3. A method of fermenting a cellulosic material, comprising: fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition in the presence of the polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.

4. A isolated man-made polynucleotide encoding the polypeptide having at least 95% sequence identity to amino acids 19 to 464 of SEQ ID NO: 2.

5. A nucleic acid construct or expression vector comprising the polynucleotide of claim 4 operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.

6. A man-made recombinant host cell comprising the polynucleotide of claim 4 operably linked to one or more control sequences that direct the production of the polypeptide.

7. A method of producing a polypeptide having cellobiohydrolase activity, comprising:

cultivating the host cell of claim 6 under conditions conducive for production of the polypeptide.

8. A man-made polynucleotide encoding a signal peptide comprising or consisting of amino acids 1 to 18 of SEQ ID NO: 2.

9. A recombinant host cell comprising a gene encoding a protein operably linked to the polynucleotide of claim 8 , wherein the gene is foreign to the polynucleotide encoding the signal peptide.

10. A method of producing a protein, comprising:

cultivating a recombinant host cell comprising a gene encoding a protein operably linked to the polynucleotide of claim 8 , wherein the gene is foreign to the polynucleotide encoding the signal peptide, under conditions conducive for production of the protein.