Use of iNOS inhibitors to increase viral yield in culture
The use of iNOS inhibitors, including aurintricarboxylic acid, dexamethasone and valproic acid, to increase the yield of a variety of viruses in culture, including recombinant herpesviruses is described.
1. A method for producing herpes simplex-1 virus (HSV-1) comprising culturing cells infected with said herpes simplex-1 virus in a cell culture medium that comprises aurintricarboxylic acid and serum, wherein the aurintricarboxylic acid is unfractionated, wherein aurintricarboxylic acid is at a concentration of about 5 μM to about 75 μM, and wherein the cells are 293 or Vero cells.
2. The method of claim 1 , wherein said HSV-1 is a wild type HSV-1.
3. The method of claim 1 , wherein said HSV-1 is a recombinant HSV-1 .
4. The method of claim 3 , wherein said recombinant HSV-1 is HSV-1 d27.1.
5. The method of claim 1 , wherein said Vero cells are V27 cells.
6. A method for producing HSV-1 d27.1 comprising:
(a) infecting V27 cells with said HSV-1 d27.1; and
(b) culturing said infected V27 cells in a cell culture medium comprising serum, and aurintricarboxylic acid, wherein aurintricarboxylic acid is at a concentration of about 5 μM to about 75 μM, and wherein aurintricarboxylic acid is unfractionated.
7. The method of claim 6 , wherein the serum is fetal bovine serum.
8. A cell culture comprising aurintricarboxylic acid, serum and 293 or Vero cells infected with HSV-1, wherein aurintricarboxylic acid is unfractionated, wherein aurintricarboxylic acid is at a concentration of about 5 μM to about 75 μM.
9. The cell culture of claim 8 wherein said Vero cells are V27 cells.