Methods for enhancing root growth of plants
The present invention relates to a method for enhancing root growth of a plant by introducing a polynucleotide encoding late embryogenesis abundant protein, group 3 (LEA3) into the plant. Plant root architecture is essential for its functions in water and nutrient uptake, anchorage and interactions with microbes in the soil.
1. A method for enhancing root growth of a plant, comprising
(i) introducing a polynucleotide encoding a group 3 late embryogenesis abundant (LEA3) protein into plant cells to obtain transformed plant cells, wherein the polynucleotide is operably linked to an expression control sequence, and wherein the LEA3 protein comprises the amino acid sequence of SEQ ID NO: 4,
(ii) regenerating transformed plants from said transformed plant cells; and
(iii) selecting from said transformed plants a transformed plant exhibiting improved root growth as compared to a non-transformed control plant.
2. The method of claim 1 , wherein the expression control sequence comprises a promoter sequence.
3. The method of claim 2 , wherein the promoter sequence is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3.
4. The method of claim 1 , wherein the transformed plant exhibits improved root growth as compared to the control plant, in the presence or absence of abscisic acid (ABA).
5. The method of claim 4 , wherein the transformed plant exhibits improved root growth as compared to the control plant in the presence of ABA at a concentration from 0.1 to 5 μM.
6. The method of claim 4 , wherein the transformed plant exhibits improved root growth as compared to the control plant in the presence of ABA at a concentration from 0.1 to 2 μM.
7. The method of claim 4 , wherein the transformed plant exhibits improved root growth as compared to the control plant in the presence of ABA at a concentration of from 0.1 to 0.5 μM.
8. The method of claim 1 , wherein the transformed plant exhibits improved root growth as compared to the control plant under nutrient deficient conditions.
9. The method of claim 1 , wherein the transgenic plant is a monocotyledon plant or a dicotyledon plant.
10. The method of claim 9 , wherein the monocotyledon plant is selected from the group consisting of rice, barley, wheat, rye, oat, corn, bamboo, sugar cane, onion, leek and ginger.
11. The method of claim 9 , wherein the plant is a monocotyledon plant, which is rice.
12. The method of claim 9 , wherein the dicotyledon plant is selected from the group consisting of Arabidopsis , eggplant, soybean, mung bean, kidney bean, pea, tobacco, lettuce, spinach, sweet potato, carrot, melon, cucumber and pumpkin.