Pretargeted activatable cell penetrating peptide with intracellularly releasable prodrug
Disclosed herein, the invention pertains to methods and compositions that find use in treatment, diagnosis, prognosis and characterization of disease and disease samples based on the ability of a disease sample to cleave a MTS molecule of the present invention. The MTS molecules of the present invention have a formula as disclosed herein and wherein A is a peptide with a sequence comprising 5 to 9 consecutive acidic amino acids, wherein the amino acids are selected from: aspartates and glutamates; B is a peptide with a sequence comprising 5 to 20 consecutive basic amino acids; X and Y are linkers; P is a pre-targeting moiety; M is a macromolecular carrier, C is a detectable moiety; and T is a compound for delivery to a target, including for example a therapeutic compound.
1. A molecule having the formula:
wherein:
P is cyclic RGD (cRGD);
M is a PEG polymer;
A is 9 consecutive glutamates;
X is selected from DPRSFL and PPRSFL;
Y is Val-Cit-(p-amido)benzyloxycarbonyl;
T is monomethyl auristatin E (MMAE);
B is 9 consecutive arginines; and
n is 1.
2. The molecule of claim 1 , wherein the molecule further comprises an imaging agent C, wherein C is Cy5.
3. The molecule of claim 1 , wherein X is DPRSFL.
4. The molecule of claim 1 , wherein X is PPRSFL.
5. A composition comprising the molecule of claim 1 and a pharmaceutically acceptable carrier vehicle.
6. A composition comprising the molecule of claim 1 and a diagnostic buffering agent.