Methods for single-molecule analysis
Methods for single-molecule preparation and analysis are disclosed herein. The methods can, for example, be used for isolating and analyzing DNA from various biological samples.
1. A method of analyzing a double-stranded DNA, the method comprising:
nicking a top strand of the double-stranded DNA at a first sequence motif with a first nickase, thereby generating a first site of nicking in the top strand;
nicking a bottom strand of the double-stranded DNA with a second nickase that recognizes the same sequence motif as the first nickase, thereby generating a second site of nicking in the bottom strand, wherein the DNA remains double-stranded adjacent to the nicks;
wherein the first site of nicking in the top strand and the second site of nicking in the bottom strand are less than 1 kb apart,
labeling the first and second sites of nicking with a first label, wherein the labeling comprises nick translation, wherein the labeling is carried out with a polymerase that leaves a flap region,
wherein said nick translation comprises extension in a direction from the first site of nicking on the top strand, and extension in an opposite direction from the second site of nicking on the bottom strand, by which a distance between the first and second nick sites increases, thereby stabilizing the double-stranded DNA;
repairing the nicks on the double-stranded DNA, wherein the repairing is carried out by a ligase, and wherein the flap region is removed to restore a ligatable nick prior to the repairing with a ligase;
nicking the repaired double-stranded DNA at a second sequence motif, wherein the repaired double-stranded DNA remains double-stranded adjacent to the nicks;
labeling the nicks at the second sequence motif on the double-stranded DNA with a second label;
repairing the nicks on the double-stranded DNA following labeling with the second label;
transporting the repaired and labeled double-stranded DNA into a nanochannel having a width of 1 nanometer to 500 nanometers and a depth of 1 nanometer to 500 nanometers;
detecting the pattern of the first label and the pattern of the second label on the double-stranded DNA in the nanochannel; and
constructing a DNA map of the double-stranded DNA, the DNA map comprising the pattern of the first label and the pattern of the second label on the double-stranded DNA in the nanochannel.
2. The method of claim 1 , further comprising marking the double-stranded DNA with a third label, wherein the third label is non-sequence-specific, and wherein the third label is different from the first label, and wherein the third label is different from the second label.
3. The method of claim 1 , further comprising modulating activity of a polymerase by adjusting the temperature, dNTP concentration, cofactor concentration, buffer concentration, or any combination thereof, during labeling.
4. The method of claim 1 , wherein the labeling is carried out in the presence of labeled dNTPs, and wherein the polymerase has a 5′ to 3′ exonuclease activity,
wherein the 5′ to 3′ exonuclease activity is performed under conditions in which at least one nucleotide is omitted, thereby removing the flap region and restoring a ligatable nick.
5. The method of claim 1 , further comprising comparing the pattern of the first label on the double-stranded DNA to a pattern of labels on a reference DNA.
6. The method of claim 1 , wherein:
the first nickase is Nb.BbvCI and the second nickase is Nt.BbvCI; or
the first nickase is Nt.BbvCI and the second nickase is Nb.BbvCI.
7. The method of claim 1 , wherein the flap region is removed with a flap endonuclease.
8. The method of claim 1 , wherein the DNA map is a physical map of the double-stranded DNA.
9. The method of claim 1 , wherein the first label comprises Atto dUTP or Alexa dUTP.
10. The method of claim 9 , wherein the labeling is carried out in the presence of dATP, dGTP, and dCTP.
11. A method of analyzing a DNA comprising:
nicking one strand of a first DNA with a first nicking endonuclease at a recognition sequence, thereby generating a first nicking site, wherein the first DNA remains double-stranded adjacent to the first nicking site;
labeling the first DNA at the first nicking site with a first label;
repairing the first nicking site on the first DNA;
nicking a complementary strand of a second DNA at the recognition sequence with a second nicking endonuclease, thereby generating a second nicking site, wherein the complementary strand of the second DNA is complementary to the one strand of the first DNA, wherein the second DNA is double stranded, wherein the second nicking endonuclease recognizes the same sequence motif as the first nicking endonuclease, and wherein the second DNA remains double-stranded adjacent to the second nicking site;
labeling the second DNA at the second nicking site with a second label, wherein the labeling is carried out with a polymerase that leaves a flap region; and wherein the first label and the second label are different;
repairing the second nicking site on the second DNA, wherein the repairing is carried out by a ligase, and wherein the flap region is removed to restore a ligatable nick prior to the repairing with a ligase;
transporting the repaired first DNA and the repaired second DNA into a nanochannel having a width of 1 nanometer to 500 nanometers and a depth of 1 nanometer to 500 nanometers;
detecting a pattern of the first label on the first DNA and a pattern of the second label on the second DNA in the nanochannel;
constructing a DNA map of the second DNA, the DNA map comprising the pattern of the second label on the double-stranded DNA in the nanochannel.
12. The method of claim 11 , further comprising marking the repaired first and second DNA with a third label, wherein the third label is non-sequence specific.
13. The method of claim 11 , wherein:
the first nicking endonuclease is Nb.BbvCI and the second nicking endonuclease is Nt.BbvCI; or
the first nicking endonuclease is Nt.BbvCI and the second nicking endonuclease is Nb.BbvCI.
14. The method of claim 11 , wherein the flap region is removed with a flap endonuclease.
15. The method of claim 11 , wherein the polymerase has a 5′ to 3′ exonuclease activity, and wherein the flap region is removed using the 5′ to 3′ exonuclease activity of the polymerase under conditions in which at least one nucleotide is limited or omitted.
16. The method of claim 15 , wherein the limited at least one nucleotide is in a concentration at least 100× less than at least one of the other nucleotides that is present.