Methods and compositions for nucleic acid sequencing
Embodiments of the present invention relate to sequencing nucleic acids. In particular, embodiments of the methods and compositions provided herein relate to preparing nucleic acid templates and obtaining sequence data therefrom.
1. A method for preparing a library of template nucleic acids to obtain sequence information from a target nucleic acid, said method comprising:
(a) contacting a template nucleic acid with a plurality of transposomes to generate a tagmented template nucleic acid, wherein at least some of the transposomes each comprise a first transposon sequence, a second transposon sequence noncontiguous with said first transposon sequence, and a transposase associated with the first transposon sequence and the second transposon sequence, wherein a plurality of transposons are inserted into the target nucleic acid;
(b) compartmentalizing the tagmented template nucleic acid comprising said plurality of inserted transposons into each vessel of a plurality of vessels, wherein the compartmentalizing comprises distributing the tagmented template nucleic acid between the plurality of vessels to provide each vessel with an amount of tagmented template nucleic acid less than about one haploid equivalent of the target nucleic acid; and
(c) removing the transposase from the template nucleic acid.
2. The method of claim 1 , wherein step (c) comprises a method selected from the group consisting of adding a detergent, changing temperature, changing pH, adding a protease, adding a chaperone, and adding a polymerase.
3. The method of claim 1 , wherein the first transposon sequence comprises a first primer site and the second transposon sequences comprise a second primer site, wherein the first primer site further comprises a first barcode and the second primer site further comprises a second barcode.
4. The method of claim 3 , wherein the first barcode and second barcode are different.
5. The method of claim 1 , wherein step (a) further comprises enriching the template nucleic acid for a sequence of interest.
6. The method of claim 1 , further comprising obtaining sequence information from the template nucleic acid.
7. The method of claim 6 , wherein the sequence information comprises haplotype sequence information.
8. The method of claim 1 , wherein the template nucleic acid is from a single cell.
9. The method of claim 1 , wherein the template nucleic acid is total genomic DNA.