A Novel EGFR Variant
The present invention features a novel EGFR variant, EGFRvVI, and methods for detecting the novel EGFR variant. The novel EGFR variant is preferentially expressed in some cancers. Methods for detecting EGFRvVI may aid in the diagnosis, prognosis, and therapeutic assessment of a subject.
1 . An isolated epithelial growth factor receptor (EGFR) protein comprising an in-frame deletion that starts in exon 3 and ends in exon 7 of wild-type EGFR.
2 . The isolated epithelial growth factor receptor (EGFR) protein of claim 1 , wherein the amino acids 90-221 have been deleted.
3 . The isolated epithelial growth factor receptor (EGFR) protein of claim 1 , wherein said EGFR protein comprises the amino acid sequence of SEQ ID NO: 5.
4 . An isolated polynucleotide coding for the epithelial growth factor receptor (EGFR), wherein the nucleotides coding for amino acids 90-221 are deleted.
5 . An isolated polynucleotide coding for the epithelial growth factor receptor (EGFR), wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO: 3 or 4.
6 . An antibody that binds to the EGFR protein of claim 1 .
7 . A method for diagnosing cancer in a subject comprising analyzing a biological sample for the presence of the EGFR protein of claim 1 or a polynucleotide encoding the EGFR protein of claim 1 , wherein presence of the EGFR protein or polynucleotide indicates the presence of a cancer or a higher predisposition of the subject to develop a cancer.
8 . The method of claim 7 , wherein the cancer is a neurologic cancer or an epithelial cancer.
9 . The method of claim 8 , wherein the neurologic cancer is glioblastoma or glioblastoma multiforme.
10 . The method of claim 8 , wherein the epithelial cancer is colon cancer, lung cancer, prostate cancer, breast cancer or other solid tumor cancers.
11 . A method for assessing the responsiveness of a subject to a therapeutic regimen comprising providing a biological sample from a subject and determining the presence, absence, or level of expression of the EGFR protein of claim 1 or a polynucleotide encoding the EGFR protein of claim 1 , wherein the presence, absence, or level of expression of the EGFR protein or polynucleotide indicates the responsiveness of the subject to the therapeutic regimen.
12 . The method of claim 11 , wherein the therapeutic regimen comprises an EGFR inhibitor.
13 . A method for recommending a therapeutic regimen comprising providing a biological sample from a subject, determining the presence of the EGFR protein of claim 1 or a polynucleotide encoding the EGFR protein of claim 1 , wherein the presence of the EGFR protein or polynucleotide rules out a therapeutic regimen comprising an EGFR inhibitor.
14 . The method of claim 12 , wherein the EGFR inhibitor is a small molecule, RNAi agent, antibody, or drug that inhibits EGFR expression or activation.
15 . A method for targeting a cancer cell that expresses the EGFR protein of claim 1 comprising administering to a subject an agent that specifically binds to the EGFR protein.
16 . The method of claim 15 , wherein said agent is an antibody.
17 . The method of claim 15 , wherein said antibody is covalently linked to a therapeutic agent.
18 . The method of claim 17 , wherein said therapeutic agent is a chemotherapeutic agent, an anti-cancer agent, a toxin, a radioisotope, or a nanoparticle.
19 . A method for treating a cancer or a symptom thereof, comprising administering to a subject an inhibitor of the EGFR of claim 1 .
20 . The method of claim 19 , wherein said inhibitor is a small molecule, RNAi agent, antibody, or drug that inhibits the expression or activation of the EGFR of claim 1 .