Phytase formulation
A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80° C.
1. A liquid enzyme formulation comprising:
(a) a phytase at a concentration of 5,000 to 50,000 unit/ml of the liquid enzyme formulation;
(b) a buffer, at a concentration of 0.5%-2.5% w/v, wherein the buffer is selected from the group consisting of: a sodium citrate, potassium citrate, citric acid, sodium acetate, acetic acid, sodium phosphate, potassium phosphate and any combination thereof;
(c) a stabilizer, at a concentration of 1%-40% w/v, wherein the stabilizer is selected from the group consisting of: a sucrose, a sorbitol, a mannitol, a glycerol, a trehalose, a sodium chloride, a sodium sulphate, or any combination thereof; and,
(d) an anti-microbial, at a concentration of 0.05%-0.40% w/v, wherein the anti-microbial is selected from the group consisting of: a potassium sorbate, sodium sorbate, a sorbic acid, a sodium benzoate, a benzoic acid, a methyl paraben, a calcium propionate, a sodium propionate, an ammonium propionate, a propionic acid, or any combination thereof,
wherein the phytase retains at least 63% of its activity at a temperature of 80° C.
2. The liquid enzyme formulation of claim 1 , wherein
(a) the buffer has a pH in a range from 4.5 to about 5.50,
(b) the stabilizer is a combination of at least two stabilizers,
(c) the stabilizer is at a concentration between 5% to 30%, or
(d) the anti-microbial is a combination of at least two anti-microbials.
3. The liquid enzyme formulation of claim 1 , wherein:
(a) the phytase has a concentration of 5,000 to 20,000 unit/ml,
(b) sodium chloride at a concentration of 1% to 20% w/v,
(c) sorbitol at a concentration of 2% to 20% w/v,
(d) glycerol at a concentration of 2% to 40% w/v,
(e) potassium sorbate at a concentration of 0.05% to 0.4% w/v,
(f) sodium benzoate at a concentration of 0.05% to 0.4% w/v,
(g) methyl paraben at a concentration of 0.05% to 0.4% w/v, or
(g) a propionate salt at a concentration of 0.05% to 0.4% w/v, wherein the propionate salt is selected from the group consisting of calcium propionate, sodium propionate, ammonium propionate, and any combination thereof.
4. The liquid enzyme formulation of claim 3 , wherein:
(a) the phytase has a concentration of 10,000 unit/ml,
(b) sodium chloride at a concentration of 15% w/v,
(c) sorbitol at a concentration of 20% w/v,
(d) glycerol at a concentration of 20% w/v,
(e) potassium sorbate at a concentration of 0.20% w/v,
(f) sodium benzoate at a concentration of 0.20% w/v,
(g) methyl paraben at a concentration of 0.20% w/v, or
(g) a propionate salt at a concentration of 0.20%, wherein the propionate salt is selected from the group consisting of calcium propionate, sodium propionate, ammonium propionate, and any combination thereof.
5. The liquid enzyme formulation of claim 1 , wherein:
(a) the phytase has a concentration of 11,000 to 12,000 unit/ml,
(b) sodium chloride at a concentration of 15% w/v,
(c) sorbitol at a concentration of 4% w/v,
(d) sodium citrate at a concentration of 50 mM,
(e) potassium sorbate at a concentration of 0.2% w/v,
(f) sodium benzoate at a concentration of 0.1% w/v, or
(g) sodium propionate at a concentration of 0.1%.
6. The liquid enzyme formulation of claim 5 , wherein the stabilizer further comprises sorbitol at a concentration of 4% w/v and/or sucrose at a concentration of 6% w/v.
7. The liquid enzyme formulation of claim 1 , wherein:
(a) the phytase has a concentration of 44,000 to 48,000 unit/ml,
(b) sucrose at a concentration of 20% w/v,
(c) sodium chloride at a concentration of 1% w/v,
(d) sodium citrate at a concentration of 50 mM,
(e) sodium benzoate at a concentration of 0.1% w/v,
(f) potassium sorbate at a concentration of 0.4% w/v, or
(g) sodium propionate at a concentration of 0.4%.
8. The liquid enzyme formulation of claim 7 , wherein the stabilizer further comprises sorbitol at a concentration of 2% to 40% w/v and/or sucrose at a concentration of 20% w/v.
9. The liquid enzyme formulation of claim 1 , wherein said liquid enzyme formulation comprises at least two of said anti-microbials.
10. The liquid enzyme formulation of claim 1 , wherein the liquid enzyme formulation is combined with an animal feed.
11. The liquid enzyme formulation of claim 10 , wherein the animal feed is a dry animal feed.