Compositions and methods for inducing apoptosis
In an aspect, the invention relates to compositions, methods, and kits for inducing apoptosis. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
1. A method of inducing apoptosis in CD20 positive human cells, the method comprising:
(i) contacting a population of human cells comprising CD20 positive cells with a first complex comprising a targeting moiety and a morpholino wherein the targeting moiety is an anti-CD20 antibody or an anti-CD20 Fab′ fragment; and
(ii) contacting the population of human cells with a second complex comprising a copolymer carrier and one or more morpholinos;
wherein the morpholino of the first complex is 5′ GAG TAA GCC AAG GAG AAT CAA TAT A 3′ (SEQ ID NO:25) and wherein the one or more morpholinos of the second complex are 5′ TAT ATT GAT TCT CCT TGG CTT ACT C 3′ (SEQ ID NO:26), wherein the contacting of the human cells with the first complex and the second complex induces apoptosis of the CD20 positive human cells.
2. The method of claim 1 , further comprising repeating step (i) and step (ii).
3. The method of claim 1 , further comprising (iii) confirming apoptosis of the human cells.
4. The method of claim 1 , wherein the cells are B-cells.
5. The method of claim 1 , wherein the cells are in a subject.
6. The method of claim 5 , wherein the subject has non-Hodgkin's lymphoma.
7. The method of claim 1 , wherein the Fab′ fragment is derived from an anti-CD20 receptor antibody.
8. The method of claim 7 , wherein the anti-CD20 antibody is 1F5, rituximab, tositumomab, ibritumomab, ofatumumab, veltuzumab, ocrelizumab, ocaratuzumab, obinutuzumab, PRO131921, BCD-020, IBI-301, ublituximab, or BLX-301.
9. A method of inducing apoptosis in CD20 positive cells, the method comprising:
contacting a population of human cells comprising CD20 positive cells with a composition comprising a first complex comprising a targeting moiety and a morpholino and a second complex comprising a complex comprising a copolymer carrier and one or more morpholinos, wherein the targeting moiety is an anti-CD20 antibody or anti-CD20 Fab′ fragment, wherein the morpholino of the first complex is 5′ GAG TAA GCC AAG GAG AAT CAA TAT A 3′ (SEQ ID NO:25) and wherein the one or more morpholinos of the second complex are 5′ TAT ATT GAT TCT CCT TGG CTT ACT C 3′ (SEQ ID NO:26), wherein the contacting of the human cells with the composition induces apoptosis of the CD20 positive cells.
10. The method of claim 9 , further comprising repeating the contacting of the human cells with the composition.
11. The method of claim 9 , wherein the cells are B-cells.
12. The method of claim 9 , wherein the cells are in a subject.
13. The method of claim 12 , wherein the subject has non-Hodgkin's lymphoma.
14. The method of claim 9 , wherein the anti-CD20 receptor antibody is 1F5, rituximab, tositumomab, ibritumomab, ofatumumab, veltuzumab, ocrelizumab, ocaratuzumab, obinutuzumab, PRO131921, BCD-020, IBI-301, ublituximab, or BLX-301.