MICROORGANISM DETECTION SENSOR, METHOD FOR MANUFACTURING SAME, AND POLYMER LAYER
The present invention is a sensor for detecting a microorganism, which is provided with a detection unit equipped with a detection electrode and a polymer layer, wherein the polymer layer is arranged on the detection electrode and is provided with a template having a three-dimensional structure complementary to a three-dimensional structure of a microorganism to be detected. The sensor detects a microorganism on the basis of the captured state of the microorganism onto the template. The polymer layer is formed by a manufacturing method including a polymerization step of polymerizing a monomer in the presence of the microorganism to be detected to form a polymer layer having the microorganism incorporated therein on the detection electrode, and a disruption step of bringing at least a part of the microorganism incorporated in the polymer layer into contact with a solution containing a lytic enzyme to disrupt the microorganism.
1 . A sensor comprising:
a detection unit equipped with a detection electrode and a polymer layer, said polymer layer being arranged on said detection electrode and being provided with a template having a three-dimensional structure complementary to a three-dimensional structure of a microorganism to be detected,
said sensor being configured to detect said microorganism on the basis of a captured state of said microorganism onto said template,
said polymer layer being formed by a manufacturing method including a polymerization step of polymerizing a monomer in the presence of a microorganism to be detected to form said polymer layer having said microorganism incorporated therein on said detection electrode, and a disruption step of bringing at least a part of said microorganism incorporated in said polymer layer into contact with a solution containing a lytic enzyme to disrupt said microorganism.
2 . The sensor according to claim 1 , wherein said solution used in said disruption step further contains a chelating agent.
3 . The sensor according to claim 1 , further comprising:
a crystal oscillator which sets said detection electrode of said detection unit to be one electrode, wherein
a captured state of said microorganism is detected by measuring a change in a mass of said polymer layer in accordance with a change in a resonance frequency of said crystal oscillator.
4 . The sensor according to claim 1 , wherein said monomer is selected from the group consisting of pyrrole, aniline, thiophene, and derivatives of those.
5 . The sensor according to claim 4 , wherein said monomer is constituted of pyrrole or its derivative.
6 . The sensor according to claim 1 , wherein a surface of said detection electrode on which said polymer layer is formed is a roughened surface.
7 . The sensor according to claim 1 , wherein said microorganism is in a state of having an excessive negative electric charge on its entirety or on its surface.
8 . A method for manufacturing a sensor for detecting a microorganism, said sensor being provided with a detection unit equipped with a detection electrode and a polymer layer, said polymer layer being arranged on said detection electrode and being provided with a template having a three-dimensional structure complementary to a three-dimensional structure of a microorganism, said method comprising:
a polymerization step of polymerizing a monomer in the presence of a microorganism to be detected to form said polymer layer having said microorganism incorporated therein on said detection electrode; and
a disruption step of bringing at least a part of said microorganism incorporated in said polymer layer into contact with a solution containing a lytic enzyme to disrupt the microorganism.
9 . A polymer layer provided with a template having a three-dimensional structure complementary to a three-dimensional structure of a microorganism, said polymer layer being manufactured by a manufacturing method including:
a polymerization step of polymerizing a monomer in the presence of said microorganism to form the polymer layer; and
a disruption step of bringing at least a part of the microorganism incorporated in said polymer layer into contact with a solution containing a lytic enzyme to disrupt the microorganism.