Identification and use of tomato genes controlling salt/drought tolerance and fruit sweetness
The present invention provides the identification and use of EGTom1 and/or EGTom2, homologs of EGTom1 and/or EGTom2, orthologs of EGTom1 and/or EGTom2, paralogs of EGTom1 and/or EGTom2, and fragments and variations thereof for altering salt tolerance, drought tolerance and/or sugar content of fruit (sweetness) in plants. The invention relates to the identification and use of nucleic acid sequences for salt/drought tolerance and fruit sweetness in plants.
1. A transgenic tomato plant, plant part, plant cell, or plant tissue culture comprising a construct comprising a nucleic acid encoding an EGTom2 polypeptide having at least 95% sequence identity to SEQ ID NO 8; wherein said transgenic tomato plant, or transgenic tomato plant produced from said plant part, plant cell, or plant tissue culture, expresses said EGTom2-encoding nucleic acid at a level of at least 300 copy/ng RNA as determined by quantitative real-time RT-PCR, and has increased fruit sweetness compared to the fruit of an untransformed control tomato plant.
2. A tomato fruit produced by the transgenic tomato plant, of claim 1 , wherein the tomato fruit comprises the nucleic acid encoding the EGTom2 polypeptide having at least 95% sequence identity to SEQ ID NO 8.
3. The transgenic tomato plant, plant part, plant cell, or plant tissue culture, of claim 1 , wherein the construct further comprises a gene termination sequence.
4. The transgenic tomato plant, plant part, plant cell, or plant tissue culture, of claim 1 , wherein the construct further comprises a promoter.
5. The transgenic tomato plant, plant part, plant cell, or plant tissue culture, of claim 1 , wherein the construct is an overexpression construct.
6. A method for producing a transgenic tomato plant with increased fruit sweetness, said method comprising:
(i) transforming a tomato plant cell with a construct comprising a nucleic acid sequence encoding a EGTom2 polypeptide having at least 95% identity to SEQ ID NO 8; and
(ii) cultivating the transgenic tomato cell under conditions conducive to regeneration and mature plant growth;
wherein the transgenic tomato plant regenerated from said transgenic plant cell expresses said EGTom2-encoding nucleic acid at a level of at least 300 copy/ng RNA as determined by quantitative real-time RT-PCR, and has increased fruit sweetness compared to the fruit of untransformed control tomato plants.
7. The method of claim 6 , wherein the construct is an over expression construct.
8. A method of producing hybrid tomato seed, said method comprising: crossing the transgenic tomato plant of claim 1 with another tomato plant, and harvesting the resultant seed.
9. An F1 hybrid plant grown from the seed produced by the method of claim 8 , wherein the F1 hybrid plant comprises the polynucleotide from the transgenic tomato plant.
10. A transgenic tomato plant of claim 1 , wherein the plant is a cultivated tomato plant ( Solanum esculentum ).
11. The transgenic tomato plant, plant part, plant cell, or plant tissue culture of claim 1 , wherein the nucleic acid sequence encodes an EGTom2 polypeptide having at least 96% sequence identity to SEQ ID No 8.
12. The transgenic tomato plant, plant part, plant cell, or plant tissue culture of claim 1 , wherein the nucleic acid sequence encodes an EGTom2 polypeptide having at least 97% sequence identity to SEQ ID No 8.
13. The transgenic tomato plant, plant part, plant cell, or plant tissue culture of claim 1 , wherein the nucleic acid sequence encodes an EGTom2 polypeptide having at least 98% sequence identity to SEQ ID No 8.
14. The transgenic tomato plant, plant part, plant cell, or plant tissue culture of claim 1 , wherein the nucleic acid sequence encodes an EGTom2 polypeptide having at least 99% sequence identity to SEQ ID No 8.