Gas-filled stabilized particles and methods of use
Provided herein are various gas-filled particles having a stabilized membrane that encapsulates the gas. Pharmaceutical compositions, methods of use and treatment, and methods of preparation are also described.
1. A method of delivering a gas to a subject in need thereof, the method comprising administering to the subject a pharmaceutical composition comprising a gas-filled particle and a pharmaceutically acceptable excipient, wherein the gas-filled particle comprises a stabilized crosslinked membrane encapsulating one or more gases, wherein the one or more gases is not a fluorinated gas, perfluorocarbon based liquid, or hemoglobin, and wherein the stabilized membrane is a crosslinked membrane, and wherein the gas-filled particle releases the encapsulated gas within a period of time of less than 1 minute following the administration, thereby delivering the gas to the subject in need thereof.
2. The method of claim 1 , wherein the pharmaceutical composition is administered to the subject.
3. The method of claim 1 , wherein the pharmaceutical composition is topically administered directly to an organ of the subject.
4. The method of claim 3 , wherein the organ is skin and a skin disorder or wound is treated.
5. The method of claim 4 , wherein the wound is a burn.
6. The method of claim 1 , wherein the subject has a neurological disease, and the pharmaceutical composition is administered in an effective amount to deliver the gas to the brain of the subject.
7. The method of claim 1 , wherein the crosslinked membrane is non-covalently crosslinked.
8. The method of claim 7 , wherein the non-covalently crosslinked membrane comprises a non-covalently crosslinked alginate.
9. The method of claim 1 , wherein the stabilized membrane comprises a polymer.
10. The method of claim 9 , wherein the polymer is a carbohydrate, a protein or a synthetic polymer, or a natural polymer.
11. The method of claim 1 , further comprising a sheath membrane between the stabilized membrane and the encapsulated gas.
12. The method of claim 1 , wherein the stabilized membrane is stabilized with one or more stabilizing agents.
13. The method of claim 1 , wherein the particle is in medium as a suspension for storage or in a powder form for storage.
14. The method of claim 13 , wherein the medium comprises a viscosity enhancing agent.
15. The method of any one of claims 1 , 2 , and 7 - 14 , wherein the composition or suspension is delivered to the subject at a flow rate of between 10 mL/min to 400 mL/min.
16. The method of any one of claims 1 , 2 , 3 - 6 , and 7 - 14 , wherein the pharmaceutical composition is formulated in a suspension, and wherein the suspension comprises at least one viscosity enhancer in an amount between 25% and 80% by weight.