Optics system for a flow cytometer
A flow cytometer includes a flow nozzle, a light source, an optics system, and a sensor analyzer. The flow nozzle provides a fluid along a flow path. The light source generates a light beam that illuminates the fluid. The optics system collects light rays that are radiated from the light beam by the fluid and passes or blocks the light rays based at least in part on the radiation angles associated with the light rays.
1. A sorting flow cytometer comprising:
a flow nozzle configured to provide a fluid that includes particles, the fluid moving along a fluid flow path, wherein the flow nozzle includes an aperture that permits only one or a small number of the particles to pass through at a time;
a light source configured to generate a light beam to illuminate the fluid;
an optics system including:
a collection optics assembly positioned adjacent the fluid flow path and aligned with the light beam path to collect light rays radiated from the light beam by the fluid, or the particles, in the fluid flow path;
a collimator arranged to receive the light rays from the collection optics assembly, wherein the collimator directs the light rays through a focus insensitive region in which positions of the light rays are independent of fluctuations in a position of the fluid flow path toward and away from the collection optics assembly; and
a filter mask positioned at the focus insensitive region to selectively filter the light rays based on radiation angles associated with the light rays; and
a sensor analyzer operable to receive and detect the light rays that pass through the filter mask;
at least two receptacles; and
control electronics configured to sort the particles into the at least two receptacles based on one or more detected characteristics of the particles.
2. The flow cytometer of claim 1 , further comprising a beam separating assembly positioned between the collimator and the filter mask that separates the light beam into at least two separate beams, and further comprising a second filter mask, wherein the filter mask and the second filter mask are positioned along the two separate beams to independently filter the separate beams according to different criteria.
3. The flow cytometer of claim 2 , wherein the separate criteria are selected from: different radiation angles, different wavelengths, and different polarizations.
4. The flow cytometer of claim 2 , wherein a light ray having a first radiation angle is blocked by the filter mask and wherein another light ray having the same first radiation angle is passed by the second filter mask.
5. The flow cytometer of claim 1 , wherein the optics system is configured to receive multiple different filter masks, including the filter mask, without requiring adjustment of the optics system for proper operation.
6. The flow cytometer of claim 2 , wherein the filter mask filters the separate beams based on different radiation angles and the second filter mask filters the separate beams based on different wavelengths.
7. The flow cytometer of claim 2 , wherein the filter mask filters the separate beams based on different radiation angles and the second filter mask filters the separate beams based on different polarizations.