Flow cytometer alignment
A sorting flow cytometer receives a current alignment of a light beam, determines a difference between the current alignment of the light beam and a target alignment of the light beam, and calculates one or more voltage values based on the difference between the current alignment and the target alignment. The sorting flow cytometer uses the voltage values to adjust a position of at least one of a first alignment actuator extending in a first axial direction and a second alignment actuator extending in a axial second direction to reduce the difference between the current alignment of the light beam and the target alignment of the light beam.
1 . An alignment module for adjusting an alignment of a light beam from a light source inside a sorting flow cytometer, the alignment module comprising:
a first alignment actuator having a fixed end and a free end extending in a first axial direction, the fixed end of the first alignment actuator configured to receive a first voltage to adjust a position of the first alignment actuator orthogonal to the first axial direction by tilting the first alignment actuator in a second axial direction based on a difference in the first axial direction between a current alignment of the light beam and a target alignment of the light beam, the second axial direction being orthogonal to the first axial direction;
a second alignment actuator having a fixed end and a free end extending in the second axial direction, and the fixed end of the second alignment actuator configured to receive a second voltage to adjust a position of the second alignment actuator orthogonal to the second axial direction by swinging the second alignment actuator in a third axial direction based on a difference in the second axial direction between the current alignment and the target alignment of the light beam;
a controller including at least one processing device and at least one memory device storing software instructions that, when executed by the at least one processing device, causes the controller to store a new alignment position based on the difference in the first axial direction and the difference in the second axial direction; and
a mirror attached to at least one of the first and second alignment actuators, the mirror configured to reflect the light beam generated from the light source.
2 . The alignment module of claim 1 , wherein the first axial direction is vertical such that the first voltage tilts the first alignment actuator up or down.
3 . The alignment module of claim 1 , wherein the second axial direction is horizontal such that the first voltage swings the second alignment actuator left or right.
4 . The alignment module of claim 1 , wherein the first and second alignment actuators are piezoelectric bending actuators.
5 . The alignment module of claim 1 , wherein the free end of the first alignment actuator is coupled to the fixed end of the second alignment actuator.
6 . The alignment module of claim 1 , wherein the controller is further configured to check alignment of the light beam after a predetermined delay.
7 . The alignment module of claim 1 , wherein the target alignment is a previous alignment of the light beam stored in at least one computer readable data storage device.