Genome editing using effector oligonucleotides for therapeutic treatment
The invention provides compositions and methods of making and using effector oligonucleotides, including effector oligonucleotides with greater than one mismatch as compared to its target sequence. These effector oligonucleotides are useful for improving the efficiency of genomic editing as well as providing therapeutic benefits to individuals in need thereof.
1. A method of making recombinant cells comprising contacting cells comprising a target sequence within a gene to be altered with an effector oligonucleotide targeted to the target sequence and comprising more than one mismatch as compared to the target sequence; and allowing the effector oligonucleotide to alter the target sequence in the cells; wherein the effector oligonucleotide comprises a sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, and SEQ ID NO:11.
2. The method of claim 1 further comprising isolating at least one of the recombinant cells to provide a substantially enriched population of recombinant cells.
3. The method of claim 1 , wherein the effector oligonucleotide matches 40 to 200 bases of the target sequence before the sequence to be deleted and matches 40 to 200 bases of the target sequence after the sequence to be deleted.
4. The method of claim 1 wherein the method further comprises contacting the cell with triplex-forming oligonucleotides or pseudocomplementary oligonucleotides.
5. The method of claim 4 wherein the triplex-forming oligonucleotide comprises a PNA.
6. The method of claim 1 , wherein the cells comprising a target sequence within a gene to be altered are selected from the group consisting of mammalian cells, human cells, animal cells, plant cells, yeast cells, insect cells, and reptilian cells.
7. The method of claim 1 , wherein the cells comprising a target sequence within a gene to be altered are stem cells.
8. The method of claim 1 , wherein the cells are selected from the group consisting of embryonic stem cells, induced pluripotent stem cells, adult stem cells, cord blood stem cells, hematopoietic stem cells, cancer stem cells, multipotent progenitor cells, lineage-restricted progenitor cells, common myeloid progenitor cells, Granulocyte-macrophage progenitor cells, megakaryocyte-erythroid progenitor cells, immune cells, differentiated immune cells and CD4-positive immune cells.
9. The method of claim 2 wherein the isolating comprises detection of the cells comprising the sequence encoded by the effector oligonucleotide by using fluorogenic oligonucleotide probes.
10. The method of claim 9 wherein the detected cells are isolated by fluorescence-activated cell sorting.
11. The method of claim 2 , further comprising separating the enriched population of recombinant cells from a growth media and resuspending the cells in a suitable media for use in cell therapy.