Compositions and method for modulating the sensitivity of plants to cytokinin
The present invention includes an expression cassette containing a polynucleotide encoding a polypeptide; and a host cell, transgenic plant (e.g., a dicot or monocot), transformed seed, and transgenic rootstock containing said expression cassette. Methods for desensitizing a plant to endogenous cytokinin; increasing seed, embryo or cotyledon size or weight; increasing the seed yield of a plant; and/or increasing the size of the root or root meristem or formation of lateral or adventitious roots are provided. In some embodiments, expression of the polypeptide is under control of a seed-preferred, embryo-preferred or root-preferred promoter.
1. An expression cassette comprising a polynucleotide encoding a polypeptide of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4 or SEQ ID NO:5, wherein said polynucleotide is operatively linked to a heterologous regulatory element.
2. An isolated host cell comprising the expression cassette of claim 1 .
3. A transgenic plant comprising the expression cassette of claim 1 .
4. The transgenic plant of claim 3 , wherein said plant is a dicot or monocot.
5. A transformed seed of the plant of claim 4 , wherein the seed comprises the expression cassette.
6. A transgenic plant comprising a transgenic rootstock, wherein the transgenic rootstock comprises the expression cassette of claim 1 .
7. A method for desensitizing a plant to endogenous cytokinin comprising introducing into a plant an expression cassette comprising a polynucleotide encoding a polypeptide of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5 and expressing the polypeptide to desensitize the plant to endogenous cytokinin.
8. A method for increasing seed, embryo or cotyledon size or weight comprising introducing into a plant an expression cassette comprising a polynucleotide encoding a polypeptide of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5 and expressing the polypeptide to increase seed, embryo or cotyledon size or weight.
9. The method of claim 8 , wherein the polynucleotide is under control of a seed-preferred or embryo-preferred promoter.
10. The method of claim 8 , wherein said method leads to an increase in yield.
11. A method for increasing the seed yield of a plant comprising introducing into a plant an expression cassette comprising a polynucleotide encoding a polypeptide of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5 and expressing the polypeptide to increase at least one of weight of seeds, number of filled seeds or harvest index, relative to a corresponding wild type plant, thereby increasing the seed yield of a plant.
12. A method for increasing the size of the root or root meristem or formation of lateral or adventitious roots comprising introducing into a plant and expression cassette comprising a polynucleotide encoding a polypeptide of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5 and expressing the polypeptide to increase the size of the root or root meristem or the formation of lateral or adventitious roots.
13. The method of claim 12 , wherein the polynucleotide is under control of a root-preferred promoter.