Protein A mutants having high alkali resistance and methods of use thereof
View Patent ↗A series of protein A mutants having high alkali resistance, and methods of using the protein A mutants are provided. The protein A mutants have a high binding affinity for regions of immunoglobulin proteins other than the complementarity determining regions. The protein A mutants can be coupled to a solid support for immunoglobulin isolation, or conjugated to a label for immunoglobulin detection. This series of protein A mutants has high chemical stability under alkaline conditions of pH 13-14, and can also be used as chromatography ligands for purification procedures that use alkaline solutions under harsh conditions, such as Clean-In-Place (CIP). Also provided are methods of immunoglobulin separation and purification, and alkali regeneration of affinity chromatography medium that uses protein A as a ligand.
1. A recombinantly expressed mutant protein A molecule for isolating, purifying, or detecting an immunoglobulin, wherein the mutant protein A molecule comprises the amino acid sequence of SEQ ID NO: 1, the amino acid sequence of SEQ ID NO: 2, the amino acid sequence of residues 7 to 54 of SEQ ID NO: 1, or the amino acid sequence of residues 7 to 54 of SEQ ID NO: 2.
2. The recombinantly expressed mutant protein A molecule of claim 1 , wherein the mutant protein A molecule is stable under alkaline conditions of pH 13-14.