Opioid detection based on high quality graphene transistor arrays and a synthetic mu receptor
The present invention provides methods for fabricating field-effect devices and sensor arrays. The field of the invention also pertains to methods of using the sensors individually, in combination, and in array fashion to detect molecules. The present invention also provides for products produced by the methods of the present invention and for apparatuses used to perform the methods of the present invention.
1. A sensing device, comprising
a graphene portion placing first and second electrodes into electronic communication with one another,
the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D peak that is less than a G peak when assessed using Raman spectroscopy,
the detection molecule being in electronic communication with the graphene and being bound to the graphene portion by non-covalent pi-pi stacking interaction between the graphene and a 1 -pyrene butanoic acid succinimidyl ester molecule and an amide bond between the 1 -pyrene butanoic acid succinimidyl ester molecule and the detection molecule, and,
the detection molecule being an integral membrane receptor molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target.
2. The sensing device of claim 1 , the sensing device being configured such that the device, during operation, is capable of detecting a change in signal related to the complementary binding between the detection molecule at the at least one target.
3. The sensing device of claim 1 , further comprising a voltage source configured to apply a voltage across the first and second electrodes.
4. A sensing device of claim 1 , wherein the detection molecule comprises a protein having an amino acid sequence that is at least about 95% identical to any one of SEQ ID 1 through SEQ ID 9.
5. A multiplexed device, comprising:
a substrate having a plurality of sensing devices according to claim 1 disposed thereon, at least two of the sensing device comprising different detection molecules.
6. The sensing device of claim 1 , wherein the detection molecule comprises protein UniProtKB: P07550 or an associated variant with the capability to bind opioid ligands.
7. A sensing device, comprising:
a graphene portion placing first and second electrodes into electronic communication with one another,
the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D peak that is less than a G peak when assessed using Raman spectroscopy,
the detection molecule being in electronic communication with the graphene,
the detection molecule being a recombinant integral membrane protein molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target.