Paint having cell wall particulate material with a protective organophosphorus esterase
Disclosed herein are novel coatings and paints comprising a biomolecule composition, wherein the biomolecule composition comprises a phosphoric triester hydrolase. Also disclosed herein are methods of detoxification of a surface contaminated with an organophosphorus compound by contacting the surface with such a coating or paint. Also disclosed herein are novel coating and paint components derived from microorganisms.
1. A paint comprising an enzymatically active esterase classified in an enzyme subclass designated by Enzyme Commission number EC 3.1.8.1;
wherein the paint comprises a cell wall material;
wherein the enzymatically active esterase comprises the aryldialkylphosphatase encoded by the gene set forth by Genbank accession number M20392 or M22863 or an enzymatically active fragment of said aryldialkylphosphatase;
wherein the aryldialkylphosphatase or fragment has catalytic activity for at least 2months after the paint has undergone solid, adherent film formation on a surface.
2. The paint of claim 1 , wherein the cell wall material is in the form of a cell fragment microorganism based particulate material.
3. The paint of claim 1 , wherein the enzymatically active esterase comprises a Co 2+ , Fe 2+ , Cu 2+ , Mn 2+ , Cd 2+, or Ni 2 +at the enzyme active site.
4. The paint of claim 1 , wherein the paint comprises one or more of a binder, a liquid component, a colorant, an additive, or a combination thereof.
5. The paint of claim 4 , wherein the binder comprises a thermoplastic binder, a thermosetting binder, or a combination thereof.
6. The paint of claim 4 , comprising an additive selected from the group consisting of an accelerator, an adhesion promoter, an antifoamer, anti-insect additive, an antioxidant, an anti-skinning agent, a buffer, a catalyst, a coalescing agent, a corrosion inhibitor, a crosslinking agent, a defoamer, a dehydrator, a dispersant, a drier, electrical additive, an emulsifier, a filler, a flame/fire retardant, a flatting agent, a flow control agent, a gloss aid, a leveling agent, a marproofing agent, a preservative, a silicone additive, a slip agent, a surfactant, a light stabilizer, a rheological control agent, a wetting additive, and combinations thereof.
7. The paint of claim 6 , wherein the additive comprises 0.001% to 20.0% by weight, of the paint.
8. The paint of claim 7 , wherein the binder comprises a polymer resin, wherein the additive comprises a combination of additives, wherein the combination of additives comprises a crosslinking agent and at least two ultraviolet light stabilizers whereby at least one light stabilizer is a sterically hindered amine, and at least one light stabilizer is a UV absorber, said UV absorber present at a concentration in excess of 5% by weight.
9. The paint of claim 8 , wherein said sterically hindered amine is bis(1,2,2,6,6,-pentamethyl-4-poperidinyl) ester or bis(2,2,6,6,-tetramethyl-1-isooctyloxy-4-piperidinyl) ester.