Methods for enhancing the degradation of cellulosic material with chitin binding proteins
The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material.
1. A method for degrading or converting a cellulosic material, comprising: treating the cellulosic material with an enzyme composition comprising a chitin binding protein and a GH61 polypeptide having cellulolytic enhancing activity, wherein the chitin binding protein is selected from the group consisting of: (a) a chitin binding protein having at least 90% sequence identity to the full-length or mature chitin binding protein of SEQ ID NO: 4; and (b) a chitin binding protein encoded by a polynucleotide having at least 90% sequence identity to the full-length or mature chitin binding protein coding sequence of SEQ ID NO: 3; wherein the combination of the chitin binding protein and the GH61 polypeptide having cellulolytic enhancing activity act synergistically compared to the chitin binding protein alone or the GH61 polypeptide alone in stimulating the degrading or converting of the cellulosic material by the enzyme composition according to the following formula with a ratio greater than 1:
CBP
-
GH
61
synergistic
effect
=
%
conversion
(
+
CBP
+
GH
61
)
%
conversion
(
+
CBP
)
+
%
conversion
(
+
GH
61
)
.
2. The method of claim 1 , wherein the chitin binding protein comprises SEQ ID NO: 4 or the mature chitin binding protein thereof.
3. The method of claim 1 , wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a cellulase, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
4. The method of claim 1 , further comprising recovering the degraded or converted cellulosic material.
5. The method of claim 4 , wherein the degraded or converted cellulosic material is a sugar.
6. The method of claim 2 , wherein the chitin binding protein consists of SEQ ID NO: 4 or the mature chitin binding protein thereof.
7. The method of claim 3 , wherein the cellulase is one or more enzymes selected from the group consisting of an endoglucanase, a cellobiohydrolase, and a beta-glucosidase.
8. The method of claim 3 , wherein the hemicellulase is one or more enzymes selected from the group consisting of a xylanase, an acetylxylan esterase, a feruloyl esterase, an arabinofuranosidase, a xylosidase, and a glucuronidase.
9. The method of claim 5 , wherein the sugar is selected from the group consisting of glucose, xylose, mannose, galactose, and arabinose.
10. The method of claim 1 , wherein the enzyme composition and/or the chitin binding protein and/or the GH61 polypeptide having cellulolytic enhancing activity are in the form of a fermentation broth with or without cells.
11. The method of claim 1 , wherein the chitin binding protein has at least 95% sequence identity to the chitin binding protein of SEQ ID NO: 4 or the mature chitin binding protein thereof.
12. The method of claim 1 , wherein the chitin binding protein has at least 96% sequence identity to the chitin binding protein of SEQ ID NO: 4 or the mature chitin binding protein thereof.
13. The method of claim 1 , wherein the chitin binding protein has at least 97% sequence identity to the chitin binding protein of SEQ ID NO: 4 or the mature chitin binding protein thereof.
14. The method of claim 1 , wherein the chitin binding protein has at least 98% sequence identity to the chitin binding protein of SEQ ID NO: 4 or the mature chitin binding protein thereof.
15. The method of claim 1 , wherein the chitin binding protein has at least 99% sequence identity to the chitin binding protein of SEQ ID NO: 4 or the mature chitin binding protein thereof.
16. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide having at least 95% sequence identity to the chitin binding protein coding sequence of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
17. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide having at least 96% sequence identity to the chitin binding protein coding sequence of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
18. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide having at least 97% sequence identity to the chitin binding protein coding sequence of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
19. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide having at least 98% sequence identity to the chitin binding protein coding sequence of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
20. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide having at least 99% sequence identity to the chitin binding protein coding sequence of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
21. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide comprising SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
22. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide consisting of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
23. The method of claim 1 , wherein the chitin binding protein has at least 91% sequence identity to the chitin protein of SEQ ID NO: 4 or the mature chitin binding protein thereof.
24. The method of claim 1 , wherein the chitin binding protein has at least 92% sequence identity to the chitin binding protein of SEQ ID NO: 4 or the mature chitin binding protein thereof.
25. The method of claim 1 , wherein the chitin binding protein has at least 93% sequence identity to the chitin binding protein of SEQ ID NO: 4 or the mature chitin binding protein thereof.
26. The method of claim 1 , wherein the chitin binding protein has at least 94% sequence identity to the chitin binding protein of SEQ ID NO: 4 or the mature chitin binding protein thereof.
27. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide having at least 91% sequence identity to the chitin binding protein coding sequence of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
28. The method of claim 1 , wherein the chitin binding protein encoded by a polynucleotide having at least 92% sequence identity to the chitin binding protein coding sequence of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
29. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide having at least 93% sequence identity to the chitin binding protein coding sequence of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.
30. The method of claim 1 , wherein the chitin binding protein is encoded by a polynucleotide having at least 94% sequence identity to the chitin binding protein coding sequence of SEQ ID NO: 3 or the mature chitin binding protein coding sequence thereof.