Use of amino acid sequences from
The present invention refers to the use of gene sequences or portions thereof characterized in that the same belong to the classes of in vitro and ex vivo induced, repressed or conserved genes in Mycobacterium tuberculosis currently infected human macrophages and to corresponding peptides or consensus peptides or proteins for the preparation of specific bio-markers for the diagnosis and prevention of active or latent disease.
1. A method for detecting a Mycobacterium tuberculosis infection in a test subject, comprising: incubating in vitro a blood sample or a lavage sample that comprises lymphocyte from the test subject with at least one isolated protein or peptide that comprises at least one Mycobacterium tuberculosis T-cell epitope, said isolated protein or peptide being an isolated protein that is selected from SEQ ID NO: 13 (Rv 0724A), SEQ ID NOS:21-22 (Rv 1251c), SEQ ID NO:24 (Rv 1478), SEQ ID NOS:70, 71 and 87 (Rv3479)
wherein said step of incubating is for a time and under conditions sufficient to stimulate the lymphocytes to produce an effector molecule; and detecting a test level of the effector molecule, wherein detection of a test level that is higher than a control level that is detected following incubation of control lymphocytes from a healthy control subject, indicates that the test subject is infected with, or has been previously exposed to, the Mycobacterium tuberculosis.
2. The method of claim 1 , wherein the subject is a human or a non human animal, and either or both of: (1) the isolated protein or peptide consists of an amino acid sequence selected from TAWITAWPGLMV (SEQ ID NO:24), AVIVRSELLTQYL (SEQ ID NO:22), GSVRQLPSVLKPPLITLRTLTLSG (SEQ ID NO:71), RPVRRVLLFWPSSGPAP (SEQ ID NO:70), GEIIFISGRLNGaa (SEQ ID NO: 13), ELMARAAVLGSAH (SEQ ID NO:21), and SALLRRLSTCPPES (SEQ ID NO:87), and (2) in the step of incubating there is present one or more Mycobacterium protein, wherein the Mycobacterium protein is selected from ESAT6, CFP 10, TB7.7 and PPD.