VMP-like sequences of pathogenic
The present invention relates to DNA sequences encoding Vmp-like polypeptides of pathogenic Borrelia , the use of the DNA sequences in recombinant vectors to express polypeptides, the encoded amino acid sequences, application of the DNA and amino acid sequences to the production of polypeptides as antigens for immunoprophylaxis, immunotherapy, and immunodiagnosis. Also disclosed are the use of the nucleic acid sequences as probes or primers for the detection of organisms causing Lyme disease, relapsing fever, or related disorders, and kits designed to facilitate methods of using the described polypeptides, DNA segments and antibodies.
1. A recombinant polypeptide molecule comprising at least 12 contiguous amino acids of SEQ ID NO: 40 immobilized on a solid support.
2. The recombinant polypeptide of claim 1 , wherein the recombinant polypeptide comprises at least 13 contiguous amino acids of SEQ ID NO: 40.
3. The recombinant polypeptide of claim 1 , wherein the recombinant polypeptide comprises at least 20 contiguous amino acids of SEQ ID NO: 40.
4. The recombinant polypeptide of claim 1 , wherein the recombinant polypeptide comprises s at least 35 contiguous amino acids of SEQ ID NO: 40.
5. The recombinant polypeptide of claim 1 , wherein the recombinant polypeptide comprises at least 50 contiguous amino acids of SEQ ID NO: 40.
6. The recombinant polypeptide of claim 1 , wherein the recombinant polypeptide comprises the sequence of SEQ ID NO: 40.
7. A method of assaying for Borrelia infection comprising:
(a) contacting a sample obtained from a subject with an isolated polypeptide, said isolated polypeptide being immobilized on a surface and comprising at least 12 contiguous amino acids of SEQ ID NO: 40; and
(b) determining whether immunologic binding occurs between the isolated polypeptide and an antibody in the sample, wherein immunologic binding is indicative of Borrelia infection.
8. The method of claim 7 , wherein said contacting step comprises performing an ELISA assay.