IDENTIFYING MARKERS OF CALORIC RESTRICTION AND CALORIC RESTRICTION MIMETICS
Markers of caloric restriction (CR) can be identified in a selected tissue by exposing an animal to CR conditions and selecting one or more genes differentially expressed in response to CR conditions in multiple subject groups. A candidate compound can be screened for likely ability to mimic the effects of CR when administered to an animal by comparing the tissue levels of expression products of the genes in animals treated with the candidate compound to those of animals subjected to CR.
1 . A probe for detecting differential expression of universal markers of CR in a tissue, comprising one of:
a) a polynucleotide that specifically hybridizes a gene listed in Tables 1 through 3 or a fragment thereof; or
b) a polypeptide binding agent that binds to a polypeptide encoded by a gene listed in Tables 1 through 6.
2 . The probe of claim 1 , wherein the tissue is heart tissue and the probes comprise polynucleotides selected from the genes listed in Table 1.
3 . The probe of claim 2 , wherein the polynucleotides are selected from genes listed in Table 4.
4 . The probe of claim 1 , wherein the tissue is skeletal muscle and the probes comprise polynucleotides selected from Table 2.
5 . The probe of claim 4 , wherein the polynucleotides are selected from genes listed in Table 5.
6 . The probe of claim 1 , wherein the tissue is white adipose and the probes comprise polynucleotides selected from Table 3.
7 . The probe of claim 6 , wherein the polynucleotides are selected from genes listed in Table 6.
8 . A kit, comprising:
a) an amplification oligonucleotide that specifically hybridizes a gene listed in Tables 1 through 6 or a fragment thereof; and
b) a labeled probe comprising a polynucleotide that specifically hybridizes a gene encoding protein listed in Tables 1 through 6 or a fragment thereof.