Reusable initiators for synthesizing nucleic acids
The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using renewable initiators coupled to a solid support. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template.
1. A method for reversibly coupling a nucleotide analog to a solid support, the method comprising:
exposing a nucleic acid initiator attached to a solid support to nucleotide analogs in the presence of a polymerase, and in the absence of a template to create a first oligonucleotide;
contacting the nucleic acid initiator with a releasing agent to release the first oligonucleotide from the nucleic acid initiator, wherein the nucleic acid initiator remains a substrate for template-independent nucleic acid synthesis after release of the first oligonucleotide.
2. The method of claim 1 , wherein the polymerase is a nucleotidyl transferase or a modified nucleotidyl transferase.
3. The method of claim 1 , wherein the polymerase is terminal deoxynucleotidyl transferase (TdT) or a modified TdT.
4. The method of claim 1 , wherein the polymerase is poly-A polymerase or a modified poly-A polymerase.
5. The method of claim 1 , wherein the polymerase is poly-U polymerase or a modified poly-U polymerase.
6. The method of claim 1 , wherein the nucleotide analogs have an unmodified 3′-OH.
7. The method of claim 1 , wherein the nucleotide analogs have an unmodified 3′-OH and a cleavable terminating group, and wherein the cleavable terminating group blocks subsequent nucleotidyl transferase activity but results in a nucleotide substrate for nucleotidyl transferase upon cleavage of the terminating group.
8. The method of claim 1 , wherein the nucleotide analogs comprise a removable blocking moiety protecting the 3′ oxygen.
9. The method of claim 1 , wherein said nucleic acid initiator comprises a ribonucleotide.
10. The method of claim 1 , wherein said nucleic acid initiator comprises an abasic deoxyribose.
11. The method of claim 1 , wherein said nucleic acid initiator comprises an abasic ribose.
12. The method of claim 1 , wherein said nucleic acid initiator comprises a non-nucleoside 5′-monophosphate.
13. The method of claim 1 , wherein said releasing agent is a type II restriction nuclease.