Methods of propagating rhinovirus C in previously unsusceptible cell lines
The present invention provides methods of propagating rhinovirus C (RV-C) in a host cell; a host cell comprising an effective amount of a heterologous CDHR3 receptor such that the host cell can support propagation of rhinovirus C; and kits comprising at least one host cell previously unable to support rhinovirus C growth, wherein the host cell comprises a heterologous CDHR3 receptor and a sample of rhinovirus C. Methods of use are also provided.
1. A method of propagating rhinovirus C or rhinovirus C variant, the method comprising the steps of:
a. obtaining a host cell comprising at least one heterologous CDHR3 receptor;
and
b. infecting the host cell with a sample of rhinovirus C or rhinovirus C variant;
wherein the rhinovirus C propagates.
2. The method of claim 1 wherein the CDHR3 receptor is a CDHR3-C 529 Y variant.
3. The method of claim 1 wherein the host cell is a cell able to support rhinovirus C replication.
4. The method of claim 3 wherein the host cell is selected from the group consisting of NCI-H358, WI-38, WisL, HEK293T, BEAS-2B, A549 and HeLa.
5. The method of claim 1 wherein the CDHR3 receptor sequence is transduced into the host cell by a viral vector.
6. The method of claim 1 wherein the CDHR3 receptor sequence is transfected into the host cell by lipofection.
7. The method of claim 1 , wherein a viral titer of at least >10e8 PFU equivalents per 10e7 cells grown in monolayer is obtained.