Drought and submergence tolerance in plants
The invention provides methods of genetically modified plants to increase tolerance to drought and/or submergence. The invention additionally provides plants having increased drought and/or submergence tolerance engineered using such methods.
1. A plant comprising a recombinant nucleic acid comprising a promoter operatively linked to a polynucleotide encoding a SAB18 polypeptide, wherein the promoter is heterologous to the polynucleotide encoding the SAB18 polypeptide, and the SAB18 polypeptide comprises the amino acid sequence of SEQ ID NO:6 or amino acids 1-439 of SEQ ID NO:2; and further, wherein the plant overexpresses the SAB18 polypeptide relative to a corresponding native plant that has not been engineered to overexpress SAB18; and is tolerant to drought or submergence.
2. The plant of claim 1 , wherein the SAB18 polypeptide comprises SEQ ID NO:6.
3. The plant of claim 1 , wherein the plant is a grass plant.
4. The plant of claim 3 , wherein the plant is a rice plant.
5. The plant of claim 1 , wherein the plant comprises a polynucleotide that expresses a Sub1A polypeptide of SEQ ID NO:5.
6. A plant cell of the plant of claim 1 .
7. A method for increasing tolerance of a plant to drought or submergence, the method comprising
introducing into plants a nucleic acid comprising a polynucleotide encoding a SAB18 polypeptide comprising an amino acid sequence at least 95% identical to SEQ ID NO:6; wherein the polynucleotide encoding the SAB18 polypeptide is operatively linked to a promoter; and
selecting a plant with increased drought tolerance or submergence tolerance compared to a plant lacking the nucleic acid.
8. The method of claim 7 , wherein the promoter is heterologous to the polynucleotide encoding the SAB18 polypeptide.
9. The method of claim 7 , wherein the SAB18 polypeptide comprises the amino acid sequence of SEQ ID NO:6 or amino acids 1-439 of SEQ ID NO:2.
10. The method of claim 7 , wherein the plant is a monocot.
11. The method of claim 10 , wherein the plant is a grass plant.
12. The method of claim 11 , wherein the grass plant is a rice plant.
13. The method of claim 7 , wherein the plant comprises a polynucleotide that expresses a Sub1A polypeptide of SEQ ID NO:5.
14. The method of claim 7 , wherein the plant comprises a polynucleotide that expresses a Sub1A polypeptide of SEQ ID NO:5; and further, wherein the selecting step selects for submergence tolerance.
15. The method of claim 14 , wherein the plant is a rice plant.
16. The method of claim 7 , wherein: (i) the SAB18 polypeptide comprises the amino acid sequence of SEQ ID NO:6 or amino acids 1-439 of SEQ ID NO:2; (ii) the plant is a grass plant that comprises a polynucleotide that expresses a Sub1A polypeptide of SEQ ID NO:5; and (iii) the selecting step selects for submergence tolerance.
17. A plant having increased tolerance to drought or to submergence produced by the method of claim 7 .