Small aperture large electrode cell
A nanopore cell includes a conductive layer and a working electrode disposed above the conductive layer and at the bottom of a well into which an electrolyte may be contained, such that at least a portion of a top base surface area of the working electrode is exposed to the electrolyte. The nanopore cell further includes a first insulating wall disposed above the working electrode and surrounding a lower section of a well, and a second insulating wall disposed above the first insulating wall and surrounding an upper section of the well, forming an overhang above the lower section of the well. The upper section of the well includes an opening that a membrane may span across, and wherein a base surface area of the opening is smaller than the at least a portion of the top base surface area of the working electrode that is exposed to the electrolyte.
1. A nanopore cell, comprising:
a conductive layer;
a working electrode disposed above the conductive layer, the working electrode forming the bottom of a well for containing an electrolyte, and wherein a top base surface area of the working electrode is configured to be exposed to the electrolyte;
a first insulating wall disposed above the working electrode and surrounding a lower section of the well; and
a second insulating wall disposed above the first insulating wall and surrounding an upper section of the well, the second insulating wall extending over the first insulating wall to form an overhang above the lower section of the well such that the upper section of the well has a smaller diameter or width than the lower section of the well, wherein the overhang extends outward and hangs over the lower section of the well, the second insulating wall defining an opening to the well that defines an area that is smaller than the top base surface area of the working electrode, wherein the opening is configured to be spanned by a membrane.
2. The nanopore cell of claim 1 , wherein the first insulating wall is differentially etchable from the second insulating wall.
3. The nanopore cell of claim 1 , wherein the second insulating wall comprises a top layer and a bottom layer, and wherein the top layer comprises a hydrophobic layer, and wherein the bottom layer comprises a hydrophilic layer.
4. The nanopore cell of claim 1 , wherein the second insulating wall comprises a top layer and a bottom layer, and wherein the top layer and the bottom layer provide two differentially functionalizable surfaces with different surface chemistry behaviors that facilitate the formation of a membrane.
5. The nanopore cell of claim 1 , wherein the working electrode comprises a planar portion that forms the bottom of the well and further comprises a vertical wall portion extending perpendicular from the planar portion along the peripheral of the planar portion, and wherein the vertical wall portion forms an electrode wall surrounding the lower section the well, and wherein the vertical wall portion is adjacent to the first insulating wall.
6. A nanopore cell, comprising:
a conductive layer;
a working electrode disposed above the conductive layer, the working electrode forming the bottom of a well, the working electrode having a first surface area configured to be exposed to an interior volume of the well; and
an insulating wall that defines the interior volume of the well in conjunction with the working electrode, the insulating wall further defining an opening to the well, wherein the opening to the well has a smaller area than the first surface area of the working electrode, and wherein the opening is configured to be spanned by a membrane.