Identification of tumour-associated cell surface antigens for diagnosis and therapy
The present invention provides agents with tumor-inhibiting activity, and which are selective for cells expressing or abnormally expressing a tumor-associated antigen. Said tumor-associated antigen has a nucleotide sequence selected from the group consisting of: (a) a nucleotide sequence selected from the specific sequences set forth herein, or a 6-50 contiguous nucleotide residue portion thereof; (b) a nucleotide sequence of a nucleic acid which hybridizes with a nucleic acid having the nucleotide sequence of (a) under stringent conditions; (c) a nucleotide sequence which is degenerate with respect to the nucleotide sequence of (a) or (b); and (d) a nucleotide sequence which is complementary to the nucleotide sequence of (a), (b) or (c). Pharmaceutical compositions and kits comprising the agents are also provided, as well as methods treating, diagnosing or monitoring a disease characterized by expression or abnormal expression of the tumor-associated antigen.
1. A method of detecting a nucleic acid which codes for a tumor-associated antigen in a biological sample comprising tissue isolated from the pancreas, stomach, breast, ovaries, prostate or esophagus of a human patient having or suspected of having a cancer disease;
wherein said nucleic acid comprises a sequence selected from the group consisting of SEQ ID NO: 9 and a sequence that is at least 90% identical to SEQ ID NO: 9; and
wherein the method comprises:
detecting the presence of the nucleic acid in the sample by polymerase chain reaction (PCR) amplification with a primer set consisting of a first primer having a sequence that can hybridize to SEQ ID NO: 9 and a second primer having a sequence that can hybridize to a nucleic acid that is complementary to SEQ ID NO: 9, which can amplify a nucleic acid fragment of SEQ ID NO: 9 under PCR conditions; and
wherein the primer set consists of SEQ ID NO: 11 and SEQ ID NO: 12.
2. The method of claim 1 , wherein said nucleic acid is detected by quantitative real time polymerase chain reaction (PCR).
3. The method of claim 1 , wherein the tumor-associated antigen comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 10, SEQ ID NO: 284, SEQ ID NO: 285, and SEQ ID NO: 286.
4. The method of claim 1 , wherein the nucleic acid which codes for the tumor-associated antigen comprises the sequence consisting of SEQ ID NO: 9.
5. The method of claim 1 , wherein the fragment amplified under PCR conditions comprises the nucleic acid sequence of SEQ ID NO: 9.
6. The method of claim 1 , wherein the biological sample comprises tissue isolated from the pancreas.
7. The method of claim 1 , wherein the biological sample comprises tissue isolated from the stomach.
8. The method of claim 1 , wherein the biological sample comprises tissue isolated from the breast.
9. The method of claim 1 , wherein the biological sample comprises tissue isolated from the ovaries.
10. The method of claim 1 , wherein the biological sample comprises tissue isolated from the prostate.
11. The method of claim 1 , wherein the biological sample comprises tissue isolated from the esophagus.