Antibacterial phage, phage peptides and methods of use thereof
The present invention is directed to the field of phage therapy for the treatment and control of bacterial infections. In particular, the present invention is directed to the novel bacteriophages F1245/05, F168/08, F170/08, F770/05, F197/08, F86/06, F87s/06 and F91a/06, isolated polypeptides thereof, compositions comprising one or more of the novel bacteriophages and/or isolated polypeptides and methods for the treatment and prevention of bacterial infection, either alone or in combination with other antibacterial therapies, e.g., antibiotics or other phage therapies.
1. A method of treating or reducing the incidence of a
Acinetobacter baumannii bacterial infection in a subject in need thereof, said method comprising administering to said subject an effective amount of a pharmaceutical composition comprising a pharmaceutically acceptable carrier; and a purified bacteriophage that has at least 99% sequence identity to the nucleotide sequence of SEQ ID NO:760 and having antibacterial activity against Acinetobacter baumannii.
2. The method of claim 1 , wherein the infection is a nosocomial infection.
3. The method of claim 1 , wherein the subject is a mammal.
4. The method of claim 1 , wherein the infection is an infection of the skin.
5. The method of claim 4 , wherein said infection of the skin is an infection associated with a diabetic foot ulcer.
6. The method of claim 4 , wherein said infection of the skin is an infection associated with an ulcer, a bed sore, a wound, or a post-operative incision.
7. The method of claim 4 , wherein the pharmaceutical composition is administered topically.
8. The method of claim 5 , wherein the pharmaceutical composition is administered topically.
9. The method of claim 6 , wherein the pharmaceutical composition is administered topically.
10. The method of claim 1 , wherein the infection is nosocomial pneumonia.
11. The method of claim 10 , wherein the pharmaceutical composition is administered by inhalation.
12. The method of claim 11 , wherein administration by inhalation uses a pump, a spray or a nebulizer.
13. The method of claim 11 , wherein the pharmaceutical composition for administration by inhalation comprises a dry powder inhaler or an aerosol spray.
14. The method of claim 1 , wherein the infection is an infection of the urinary tract.
15. The method of claim 14 , wherein the pharmaceutical composition is administered by a catheter.
16. The method of claim 1 , wherein the infection is an infection of the cerebrospinal fluid or is nosocomial meningitis.
17. The method of claim 1 , wherein the infection is an infection of the peritoneal fluid.
18. The method of claim 1 , wherein the infection is an infection associated with a surgical drain, an infection associated with catheterization, or an infection associated with continuous ambulatory peritoneal dialysis (CAPD).
19. The method of claim 1 , further comprising administering to said subject an antibiotic for treating infection by Acinetobacter baumannii or an additional bacteriophage known to have antibacterial or antimicrobial activity against Acinetobacter baumannii.
20. The method of claim 1 , wherein the purified bacteriophage has the nucleotide sequence of SEQ ID NO:760.