Materials and method for modifying a biochemical component in a plant
View Patent ↗A method of modifying the amount of at least one biochemical component in a plant comprising expressing Qua-Quine Starch (QQS) in the plant, the wild-type of which does not express QQS; a transgenic plant, or part thereof, which comprises and expresses QQS as a transgene and in which the amount of at least one biochemical component is modified; a tissue culture of regenerable cells of the transgenic plant; a vector comprising a nucleotide sequence, which encodes the coding sequence of QQS, operably linked to a non-native promoter, which promotes expression of the nucleotide sequence in a plant, which is other than Arabidopsis ; and a method of producing a food or industrial product from a plant.
1. A transgenic plant or part thereof which comprises and expresses a non-native Qua-Quine Starch (QQS) protein that has at least 90% identity to SEQ ID NO: 2 and in which at least the protein content is higher than the protein content in a corresponding wild-type (WT) plant or corresponding WT part thereof which does not express the QQS protein.
2. The transgenic plant or part thereof of claim 1 , wherein the QQS protein is an Arabidopsis QQS protein.
3. The plant or part thereof of claim 2 , wherein the plant is soybean, rice, or corn.
4. A tissue culture of regenerable cells of the transgenic plant of claim 2 , wherein the regenerable cells comprise and express the Arabidopsis QQS.
5. The plant or part thereof of claim 3 , wherein the Arabidopsis QQS protein is encoded by a nucleotide sequence under the control of a non-native promoter.
6. The plant or part thereof of claim 5 , wherein the non-native promoter is a constitutive promoter.
7. The plant or part thereof of claim 6 , wherein the constitutive promoter is a cauliflower mosaic virus 35S promoter.
8. The plant or part thereof of claim 5 , wherein the non-native promoter is an inducible promoter.
9. The plant or part thereof of claim 5 , wherein the non-native promoter is a developmentally specific promoter.
10. The plant or part thereof of claim 9 , wherein the developmentally specific promoter is a seed-specific promoter.
11. The plant or part thereof of claim 5 , wherein the non-native promoter is a synthetic promoter.
12. The plant or part thereof of claim 11 , wherein the synthetic promoter is a hybrid promoter.
13. The plant or part thereof of claim 3 , wherein the amount of protein in the seeds of soybean is increased by at least 30% as compared to the amount of protein in the seeds of wild-type (WT) soybean.
14. The plant or part thereof of claim 3 , wherein the amount of protein in the seeds of soybean is increased by at least 45% as compared to the amount of protein in the seeds of WT soybean.
15. The plant or part thereof of claim 3 , wherein the amount of protein in the seeds of soybean is increased by at least 60% as compared to the amount of protein in the seeds of WT soybean.
16. A tissue culture of regenerable cells of the transgenic plant of claim 3 , wherein the regenerable cells comprise and express the Arabidopsis QQS protein.
17. The transgenic plant or part thereof of claim 2 , wherein the Arabidopsis QQS protein comprises the amino acid sequence of SEQ ID NO: 2.
18. The transgenic plant or part thereof of claim 2 , wherein the amount of protein in the transgenic plant or part thereof is increased by at least 15% as compared to the amount of protein in the corresponding WT plant or corresponding WT part thereof.
19. The transgenic plant or part thereof of claim 2 , wherein the amount of protein in the transgenic plant or part thereof is increased by at least 10% as compared to the amount of protein in the corresponding WT plant or corresponding WT part thereof.
20. The transgenic plant or part thereof of claim 2 , wherein the amount of protein in the transgenic plant or part thereof is increased by at least 5% as compared to the amount of protein in the corresponding WT plant or corresponding WT part thereof.