Modulating bacterial MAM polypeptides in pathogenic disease
View Patent ↗The present invention relates to methods and compositions for preventing or inhibiting pathogenic bacterial infections in a subject caused by pathogenic bacteria expressing a MAM polypeptide by administering to a subject a composition comprising a MAM polypeptide or a non-pathogenic bacterium expressing a MAM polypeptide, or a combination thereof.
1. A method for identifying or quantifying protein-lipid interaction on a membrane comprising contacting a membrane with a peptide or polypeptide comprising multivalent adhesion molecule (MAM) mce repeat region.
2. The method of claim 1 , wherein said peptide comprises 1, 2, 3, 4, 5, 6 or 7 mce repeat regions.
3. The method of claim 1 , wherein said peptide or polypeptide is labeled.
4. The method of claim 3 , wherein said label is a fluorescent label, a chemilluminscent label, and enzyme label, a dye, a colorimetric label, a quantum dot, or a ligand.
5. The method of claim 1 , wherein said membrane is part of an intact cell.
6. The method of claim 5 , wherein said cell is a fixed cell.
7. The method of claim 5 , wherein said cell is a living cell.
8. The method of claim 7 , wherein said cell has been treated with an agent.
9. The method of claim 8 , further comprising the step, prior to treatment with said agent, of identifying or quantifying lipid on said cell's membrane.