Nanospheres encapsulating bioactive material and method for formulation of nanospheres
A method of transdermal delivery of a vaccine, comprising preparing microparticles of encapsulated vaccine by spray drying a mixture of the vaccine and at least one polymer, and injecting the microparticles transdermally using a microneedle delivery apparatus.
1. A method of transdermal delivery of a vaccine, comprising:
(a) preparing a polymeric solution comprising a nonorganic solvent, and at least two polymers selected from the group consisting of hydroxyl-propyl methylcellulose acetate succinate, cellulose acetate phthalate polymer (CAP), ethyl cellulose, HPMC-AS, Eudragit® FS30D, and chitosan glycol;
(b) preparing microparticles of encapsulated vaccine by spray drying at a temperature higher than the boiling point of the solvent and lower than the temperature at which the vaccine degrades a mixture of a proteinaceous vaccine material and the polymeric solution;
(c) providing at least one microneedle;
(d) introducing the microparticles into the at least one microneedle; and,
(e) injecting the microparticles transdermally through the at least one microneedle whereby the encapsulated vaccine retains bioactivity after cellular uptake.