Methods for using polypeptides having cellobiohydrolase activity
View Patent ↗The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A method for degrading or converting a cellulosic material, said method comprising:
(i) treating the cellulosic material with an enzyme composition, wherein the enzyme composition comprises a polypeptide having cellobiohydrolase activity; and
(ii) recovering the degraded or converted cellulosic material;
wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 92% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
2. The method of claim 1 , wherein the cellulosic material is pretreated.
3. A method for producing a fermentation product, said method comprising:
(a) saccharifying a cellulosic material with an enzyme composition, wherein the enzyme composition comprises a polypeptide having cellobiohydrolase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation;
wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 92% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
4. The method of claim 3 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.
5. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity further comprises a cellulose binding domain.
6. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity is a variant of the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2, wherein the variant is modified by a substitution, deletion, and/or insertion of one or more amino acids in the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2, and wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 92% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
7. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
8. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 97% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
9. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 98% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
10. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 99% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
11. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
12. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises or consists of the amino acid sequence of SEQ ID NO: 2.
13. The method of claim 3 , wherein the polypeptide having cellobiohydrolase activity further comprises a cellulose binding domain.
14. The method of claim 3 , wherein the polypeptide having cellobiohydrolase activity is a variant of the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2, wherein the variant is modified by a substitution, deletion, and/or insertion of one or more amino acids in the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2, and wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 92% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
15. The method of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
16. The method of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 97% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
17. The method of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 98% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
18. The method of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence that has at least 99% sequence identity to the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
19. The method of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises the amino acid sequence of amino acids 19 to 455 of SEQ ID NO: 2.
20. The method of claim 3 , wherein the polypeptide having cellobiohydrolase activity comprises or consists of the amino acid sequence of SEQ ID NO: 2.