Method of producing a polymer matrix
The present invention relates to a biomimetic matrix for providing structural support and scaffolding that allows for regeneration of dentin, pulp, and periodontal tissues. A method of making the biomimetic matrix provides the ability to select both a size of a pore or tubule formed in the biomimetic matrix and a density of pores or tubules disposed throughout the biomimetic matrix. The present invention discloses an approach of successful tubular dentin regeneration both in vitro and in vivo using the biomimetic matrix.
1. A method of producing a polymer matrix, the method comprising:
electrospinning a liquefied polymer onto an electrode to create a nanofibrous layer having a porosity; and
ablating, via a laser, the layer to form a tubule through the layer; and
wherein a diameter of the tubule changes along a depth of the nanofibrous layer.
2. The method of claim 1 wherein the electrode is a rotating mandrel.
3. The method of claim 1 wherein, the liquefied polymer is a biocompatible melted polymer.
4. The method of claim 1 , wherein the porosity of the nanofibrous layer changes along a depth of the nanofibrous layer.
5. The method of claim 1 , wherein the diameter of the tubule on a top surface of the polymer matrix is smaller than a diameter of the tubule on a bottom surface of the nanofibrous layer.
6. The method of claim 1 , wherein the diameter changes in a contiguous manner from a first surface of the polymer matrix to a bottom surface of the polymer matrix.