Multiple arm apparatus and methods for separation of particles
The invention provides apparatus for separation of particles and methods for using the apparatus. In an embodiment, the apparatus includes three arms extending radially from a central reservoir, each arm being associated with a separation electrode. At least one on the arms includes a separation medium. Using a sequence of driving and mobility-changing voltages, target particles can be separated from closely related particles within a sample. For example, single point mutations can be resolved from a sample containing predominantly wild type nucleic acids.
1. An apparatus for separating particles comprising:
at least three electrodes circumferentially surrounding a central reservoir;
a separation medium between at least one electrode and the central reservoir;
an arm between each of the at least three electrodes and the central reservoir, at least one of the arms comprising the separation medium, wherein the arm comprising the separation medium comprises a loading reservoir adjacent the separation medium and the loading reservoir extends perpendicularly relative to the length of the separation arm; and
a loading buffer chamber adjacent the separation medium and opposite the loading reservoir.
2. The apparatus of claim 1 , wherein the central reservoir comprises a buffer or an additional separation medium.
3. The apparatus of claim 1 , wherein the separation medium comprises an affinity agent that has a binding affinity for a target particle.
4. The apparatus of claim 1 , further comprising a controller for regulating the voltage applied to each electrode.
5. The apparatus of claim 1 , wherein each arm extends radially outwardly from the central reservoir.
6. The apparatus of claim 5 , wherein each arm is symmetrically spaced around the central reservoir.
7. The apparatus of claim 1 , wherein two arms comprise the separation medium.
8. The apparatus of claim 1 , wherein each arm comprises the separation medium.
9. The apparatus of claim 1 , comprising a first loading electrode in the loading reservoir and a second loading electrode in the loading buffer chamber.
10. The apparatus of claim 1 , further comprising a buffer chamber at the distal end of each separation arm, each of the at least three electrodes being located in a respective buffer chamber.
11. A cassette comprising the apparatus of claim 1 , the cassette comprising a top plate and a bottom plate, wherein the arms interpose the top and bottom plates and the separation medium is between the top plate and the bottom plate.