THERMOPHILIC AND THERMOACIDOPHILIC METABOLISM GENES AND ENZYMES FROM ALICYCLOBACILLUS ACIDOCALDARIUS AND RELATED ORGANISMS, METHODS
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering metabolism in a cell using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
1 . An expression vector comprising an isolated polynucleotide encoding a polypeptide having at least 90% sequence identity to SEQ ID No. 392.
2 . The expression vector of claim 1 , wherein the expression vector comprises a polynucleotide having at least 90% identity to SEQ ID No. 393.
3 . The expression vector of claim 1 , wherein the encoded polypeptide has Acetyl-CoA C-acetyltransferase enzymatic activity.
4 . A method of modulating or altering metabolism in a cell, the method comprising:
providing the expression vector of claim 3 to the cell and expressing the encoded polypeptide in the cell.
5 . The method according to claim 4 , further comprising glycosylating, or otherwise post-translationally modifying the encoded peptide in the cell.
6 . An expression vector comprising an isolated polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID No. 392.
7 . The expression vector of claim 6 , wherein the encoded polypeptide has Acetyl-CoA C-acetyltransferase enzymatic activity.
8 . The expression vector of claim 6 , wherein the expression vector comprises a polynucleotide having at least 95% identity to SEQ ID No. 393.