Hydroxyapatite powder and process for producing same, composition based on this powder and process for preparing same and kit comprising this powder
The present invention relates to a hydroxyapatite and/or tricalcium phosphate powder characterized in that it has undergone at least one sintering step at a temperature between 400° C. and 600° C. The invention also relates to a process for preparing such a powder, and to a composition comprising such a powder for use as an anti-tumour auto-vaccine and particularly in the treatment of the following pathological conditions: osteosarcoma, B or T lymphoma, mammary tumour, melanoma, haemangiosarcoma, mastocytoma, fibrosarcoma, brain tumours and schwannoma in a subject. The present invention also covers a drug combination comprising the composition of the invention and at least one second therapeutic agent, preferably an anti-tumour agent and/or a radiotherapeutic agent.
1. A method for treating B cell lymphomas in a subject comprising the step of administering to a subject suffering from B cell lymphomas an antitumor auto-vaccine composition, wherein the composition comprises a hydroxyapatite or tricalcium phosphate powder on which cytoplasmic proteins extracted from a tumor sample from said subject have been absorbed, said powder having a specific surface area SS≥m 2 /g and a particle size G≤200 μm, and being suspended in an injection liquid.
2. The method of claim 1 , wherein the powder has undergone a sintering step between 400° C. and 600° C.
3. The method of claim 1 , wherein the powder has a particle size G≤25 μm.
4. The method of claim 1 , wherein the powder has a particle size G between 25 μm and 45 μm.
5. The method of claim 1 , wherein the X-ray diffraction spectrum of the powder has a ratio of the width at half maximum to total height of the line, with an angle 2θ value equal to 31.773 degrees+/−1%, of between 0.2% and 0.35%.
6. The method of claim 1 , wherein the subject is a human.
7. The method of claim 1 , wherein the subject is a dog.
8. The method of claim 1 , wherein the composition is administered in combination with an antitumor agent or a radiotherapeutic agent.
9. The method of claim 1 , wherein one dose administered by injection to the subject comprises between 30 mg and 50 mg of hydroxyapatite or tricalcium phosphate and between 1000 μg and 2000 μg of proteins.