Human Adaptation of H7 HA
The present invention provides, among other things, systems (e.g., comprising compositions and/or methods) for diagnosis, prophylaxis, treatment, prevention, and/or characterization of influenza transmission and/or infection. The present invention also provides methods for monitoring influenza variants for their potential to present a pandemic risk to humans.
1. A polypeptide whose amino acid sequence includes a sequence element that:
i) at least 95% identical to at least ten continuous amino acids of a reference H7 HA polypeptide selected from the group consisting of SEQ ID NO: 17-38, 43, 45-47, 56-58 and 60-65, wherein the at least ten continuous amino acids includes amino acid position 228; and
ii) has a serine residue at its position corresponding to the reference H7 polypeptide amino acid position 228.
2. The polypeptide of claim 1 , wherein the polypeptide further comprises approximately residues 50-220 of the reference H7 HA polypeptide.
3. The polypeptide of claim 1 , wherein the portion further includes amino acid position 226, and wherein the sequence element has a leucine or isoleucine residue at its position corresponding to the reference H7 HA polypeptide position 226.
4. The polypeptide of claim 1 , wherein the polypeptide further includes amino acid position 122, and wherein the sequence element has an alanine residue at its position corresponding to the reference H7 HA polypeptide position 122.
5. The polypeptide of claim 1 , wherein the polypeptide further includes amino acid position 174, and wherein the sequence element has a serine residue at its position corresponding to the reference H7 HA polypeptide position 174.
6. The polypeptide of claim 1 , wherein the polypeptide further includes amino acid position 186, and wherein the sequence element has a valine residue at its position corresponding to the reference H7 HA polypeptide position 186.
7. The polypeptide of claim 1 , wherein the polypeptide further includes amino acid position 202, and wherein the sequence element has a valine residue at its position corresponding to the reference H7 HA polypeptide position 202.
8. The polypeptide of claim 1 , wherein the polypeptide further includes at least one amino acid position selected from the group consisting of 122, 174, 186, 202, 226, and combinations thereof, and wherein the sequence element has:
alanine residue at its position 122;
serine residue at its position 174;
valine residue at its position 202;
leucine or isoleucine residue at its position 226;
corresponding to the reference H7 HA polypeptide.
9. The polypeptide of claim 1 , wherein the polypeptide is between 100 amino acids and 600 amino acids in length.
10. The polypeptide of claim 1 , wherein the polypeptide is between 200 amino acids and 600 amino acids in length.
11. A nucleic acid that encodes the polypeptide of claim 1 .
12. A cell line that expresses the polypeptide of claim 1 .
13. The polypeptide of claim 1 , wherein the polypeptide further has an amino acid sequence that includes L226, I226, S174, A122, V186, V202, or combinations thereof.
14. A vaccine composition comprising at least one polypeptide whose amino acid sequence includes a sequence element that:
i) at least 95% identical to at least ten continuous amino acids of a reference H7 HA polypeptide selected from the group consisting of SEQ ID NO: 17-38, 43, 45-47, 56-58 and 60-65, wherein the at least ten continuous amino acids includes amino acid position 228; and
ii) has a serine residue at its position corresponding to the reference H7 polypeptide amino acid position 228;
and a pharmaceutically acceptable carrier.
15. A polypeptide whose amino acid sequence includes a sequence element that:
i) at least 80% identical to at least ten continuous amino acids of a reference H7 HA polypeptide selected from the group consisting of SEQ ID NO: 17-38, 43, 45-47, 56-58 and 60-65, wherein the at least ten continuous amino acids includes amino acid position 228; and
ii) has a serine residue at its position corresponding to the reference H7 polypeptide amino acid position 228.
16. A nucleic acid that encodes the polypeptide of claim 15 .
17. A cell line that expresses the polypeptide of claim 15 .
18. The polypeptide of claim 15 , wherein the polypeptide further has an amino acid sequence that includes L226, I226, S174, A121, V186, V202, or combinations thereof.
19. A vaccine composition comprising, the polypeptide of claim 15 .