Compositions and methods for use in medical diagnosis
The present invention relates to compositions and methods for use in medical diagnosis and patient monitoring. It more particularly relates to a biocompatible gel comprising nanoparticles and/or nanoparticle aggregates, wherein: i) the nanoparticles and/or nanoparticles of said aggregate comprise an inorganic material comprising at least one metal element having an atomic number Z of at least 25, each of said nanoparticles and nanoparticle aggregates being covered with a biocompatible coating; ii) the nanoparticles' and/or nanoparticle aggregates' concentration is of about or less than 0.5% (w/w); and iii) the apparent viscosity at 2 s −1 of the gel comprising the nanoparticles and/or nanoparticle aggregates is between about 0.1 Pa·s and about 1000 Pa·s when measured between 20° C. and 37° C. The composition of the invention typically allows the delineation and visualization of at least 40% of the target biological tissue when said tissue is observed using an X-ray imaging equipment.
1. A method for delineating and visualizing at least 50% of a tumor bed in a subject, wherein the tumor bed is the tissue covering the cavity obtained following tumor resection in the subject, and wherein the method comprises a step a) of depositing on the tumor bed in the subject a biocompatible gel comprising nanoparticles and/or nanoparticle aggregates wherein: i) the nanoparticles and/or nanoparticles of the aggregate comprise hafnium (IV) oxide (HfO 2 ) or comprise gold and hafnium (IV) oxide (HfO 2 ), each of said nanoparticles and/or said nanoparticle aggregate being covered with a biocompatible coating; ii) the concentration of the nanoparticle and/or nanoparticle aggregates in the biocompatible gel is about or less than 0.5% (w/w); iii) the biocompatible gel comprises cellulose, methylcellulose, carboxymethylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, or hyaluronic acid; and iv) the apparent viscosity at 2 s −1 of the biocompatible gel comprising nanoparticles and/or nanoparticle aggregates is between about 1 Pa·s and about 750 Pa·s when measured between 20° C. and 37° C. and the gel is deposited in a layer having a thickness between 100 μm and 0.5 cm, wherein the nanoparticles and/or the nanoparticle aggregates of said gel delineate the tumor bed, and a step b) of visualizing at least 50% of the delineated tumor bed in the subject using X-ray imaging equipment.
2. The method according to claim 1 , wherein the concentration of the nanoparticle and/or nanoparticle aggregates in the biocompatible gel is between about 0.15% and about 0.5% (w/w).
3. The method according to claim 1 , wherein the nanoparticles and/or nanoparticle aggregates comprise hafnium oxide (HfO 2 ).
4. The method according to claim 1 , wherein the nanoparticle or nanoparticle aggregate further comprises at least one targeting agent.
5. The method according to claim 1 , wherein the X-ray imaging equipment is a CT scanner.
6. The method according to claim 1 , wherein the nanoparticles and/or nanoparticle aggregates comprise hafnium (IV) oxide (HfO 2 ) and gold.
7. The method according to claim 1 , comprising delineating and visualizing at least 70% of the tumor bed in the subject.
8. The method according to claim 1 , comprising delineating and visualizing at least 80% of the tumor bed in the subject.
9. The method according to claim 1 , wherein the step of delineating and visualizing the tumor bed is performed between 24 hours and two weeks after the step of depositing on the tumor bed in the subject the biocompatible gel comprising nanoparticles and/or nanoparticle aggregates.
10. The method according to claim 1 , wherein the biocompatible gel is selected from methylcellulose, carboxymethylcellulose, hydroxyethylcellulose and hydroxypropylcellulose.
11. The method according to claim 1 , wherein the nanoparticles comprise gold metal covered with hafnium oxide.