Transgenic Expression Of Archaea Superoxide Reductase
This invention provides a stably transformed plant, plant part and/or plant cell, comprising a heterologous polynucleotide encoding a superoxide reductase (SOR) from an archaeon species, wherein said stably transformed plant, plant cell, and/or plant part has increased disease resistance. The invention further provides a method of increasing disease resistance in a plant, plant cell, or plant part, comprising: introducing into said plant, plant cell, or plant part a heterologous polynucleotide encoding a superoxide reductase from an archaeon species to produce a stably transformed plant, plant cell, or plant part, thereby producing a plant, plant part, or plant cell having increased disease resistance as compared to a control. Additionally provided are plants, plant parts, and plant cells produced by the methods of the invention, as well as progeny and products produced therefrom.
1 . A stably transformed plant, plant part and/or plant cell, comprising a heterologous polynucleotide encoding a superoxide reductase (SOR) from an archaeon species, wherein said stably transformed plant, plant cell, and/or plant part has increased disease resistance.
2 . The stably transformed plant, plant part and/or plant cell of claim 1 , wherein said superoxide reductase is localized to the cytosolic membrane, the chloroplast, the peroxisome, the cell wall, and/or mitochondria of said stably transformed plant, plant cell, and/or plant part.
3 . The stably transformed plant, plant part and/or plant cell of claim 2 , wherein said superoxide reductase is a membrane associated protein of the chloroplast, the peroxisome, the cell wall, and/or mitochondria.
4 . The stably transformed plant, plant part or plant cell of claim 1 , wherein the archaeon species is a species from the genus Pyrococcus , a species from the genus Thermococcus , or a species from the genus Archaeoglobus.
5 . The stably transformed plant, plant part or plant cell of claim 4 , wherein the archaeon species is Pyrococcus furiosus.
6 . The stably transformed plant, plant part or plant cell of claim 5 , wherein the heterologous polynucleotide encoding a SOR from an archaeon species comprises a nucleotide sequence having substantial identity to the nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:2 or encodes an amino acid sequence having substantial identity to an amino acid sequence of SEQ ID NO:3 or SEQ ID NO:4.
7 . (canceled)
8 . The stably transformed plant, plant part or plant cell of claim 1 , wherein the plant, plant part, or plant cell, further comprises a heterologous polynucleotide encoding a CO 2 transporter.
9 - 10 . (canceled)
11 . The stably transformed plant, plant part or plant cell of claim 1 , wherein the superoxide reductase and/or CO 2 transporter are each operably associated in fusion with a targeting sequence.
12 . A seed of the stably transformed plant of claim 1 , wherein the seed comprises in its genome a heterologous polynucleotide encoding superoxide reductase from an archaeon species and optionally a heterologous polynucleotide encoding a CO 2 transporter.
13 - 14 . (canceled)
15 . A product produced from the stably transformed plant, plant part, or plant cell of claim 1 , optionally wherein the product is biofuel, food, drink, animal feed, fiber, and/or pharmaceuticals.
16 . (canceled)
17 . A stably transformed plant, plant part, plant cell, yeast cell or bacterial cell comprising a first heterologous polynucleotide encoding a superoxide reductase (SOR) from an archaeon species and a second heterologous polynucleotide encoding a CO 2 transporter.
18 . (canceled)
19 . The stably transformed plant, plant part, plant cell, yeast cell or bacterial cell of claim 17 , wherein the archaeon species is a species from the genus Pyrococcus , a species from the genus Thermococcus , or a species from the genus Archaeoglobus.
20 . The stably transformed plant, plant cell, plant part, yeast cell or bacterial cell of claim 17 , wherein the archaeon species is Pyrococcus furiosus.
21 . The stably transformed plant, plant cell, plant part, yeast cell or bacterial cell of claim 17 , wherein the first heterologous polynucleotide encoding a SOR from an archaeon species comprises a nucleotide sequence having substantial identity to the nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:2.
22 . The stably transformed plant, plant part, plant cell, yeast cell or bacterial cell of claim 17 , wherein the second heterologous polynucleotide encoding a CO 2 transporter is:
(a) a nucleotide sequence having substantial identity to the nucleotide sequence of SEQ ID NO:58, SEQ ID NO:60 or SEQ ID NO:62 and/or
(b) a nucleotide sequence that encodes an amino acid sequence having substantial identity to an amino acid sequence of SEQ ID NO:59, SEQ ID NO:61 and/or SEQ ID NO:62.
23 - 25 . (canceled)
26 . The stably transformed plant, plant part, plant cell, yeast cell or bacterial cell, of claim 17 , wherein the superoxide reductase and/or CO 2 transporter are each operably associated (in fusion) with a targeting sequence.
27 . A method of increasing disease resistance in a plant, plant cell, or plant part, comprising:
introducing into said plant, plant cell, or plant part a heterologous polynucleotide encoding a superoxide reductase from an archaeon species to produce a stably transformed plant, plant cell, or plant part, thereby producing a plant, plant part, or plant cell having increased disease resistance as compared to a control.
28 . The method of claim 27 , wherein said superoxide reductase is expressed and localized to the chloroplast, the cell wall, mitochondria and/or as a membrane associated protein of said stably transformed plant, plant cell, or plant part.
29 . The method of claim 27 , wherein the archaeon species is Pyrococcus furiosus.
30 . The method of claim 29 , wherein the heterologous polynucleotide encoding a superoxide reductase from Pyrococcus furiosus is a nucleotide sequence having substantial identity to a nucleotide sequence of SEQ ID NO:1 and/or SEQ ID NO:2, and/or encodes an amino acid sequence having substantial identity to an amino acid sequence of SEQ ID NO:3 and/or SEQ ID NO:4.
31 . (canceled)
32 . The method of claim 27 , wherein the plant, plant cell, plant part, yeast cell or bacterial cell further comprises a heterologous polynucleotide encoding a polypeptide having the enzyme activity of a CO 2 transporter
33 - 34 . (canceled)
35 . A stably transformed plant, plant cell and/or plant part produced by the method of claim 27 .
36 . A seed of the stably transformed plant of claim 35 , wherein the seed comprises in its genome a heterologous polynucleotide encoding a superoxide reductase from an archaeon species and optionally a heterologous polynucleotide encoding a CO 2 transporter.
37 . (canceled)
38 . A product produced from the stably transformed plant, plant cell, or plant part of claim 35 or the seed of claim 36 , optionally wherein the product is biofuel, food, drink, animal feed, fiber, and/or pharmaceutical.
39 . (canceled)