Self-digitization of sample volumes
Devices, systems and apparatuses for the discretization and manipulation of sample volumes are provided. Related methods are also provided.
1. A microfluidic device, comprising:
a disc-shaped body comprising a center, a center region, an outer edge, and a radius from the center to the outer edge, the disc-shaped body having a central axis and further comprising:
a fluid inlet port located in the center region of the disc-shaped body;
a flow channel having a proximal end near the center region, a distal end near the outer edge, the flow channel in fluidic communication with the fluid inlet port, wherein the flow channel is substantially straight and continuous from the proximal end to the distal end, and wherein the proximal end and the distal end define a flow axis of the flow channel;
a plurality of fluidic harbors, wherein each fluidic harbor of the plurality of fluidic harbors is directly connected to the flow channel and offset from the flow axis of the flow channel;
a plurality of drainage channels, wherein each drainage channel of the plurality of drainage channels is directly connected to and connects a fluidic harbor of the plurality of fluidic harbors and the flow channel; and
a fluid outlet port in fluidic communication with the distal end of the flow channel, wherein the fluid outlet port is located in the center region and closer to the central axis of the disc-shaped body than the distal end of the flow channel, and wherein the fluidic communication between the distal end of the flow channel and the fluid outlet port comprises a channel,
wherein the center region comprises a portion of the disc-shaped body from the center to 80% or less of the radius of the disc-shaped body.
2. The microfluidic device of claim 1 , further comprising a flow cell, wherein the flow cell comprises the fluid inlet port, the fluid outlet port, and a plurality of the flow channels, wherein each of the flow channels is in fluidic communication with the fluid inlet port and the fluid outlet port.
3. The microfluidic device of claim 2 , further comprising a common fluid reservoir, wherein the common fluid reservoir is directly connected to the distal end of each of the flow channels and wherein the common fluid reservoir is separately directly connected to the fluid outlet port.
4. The microfluidic device of claim 2 , further comprising a plurality of the flow cells.
5. The microfluidic device of claim 2 , wherein the plurality of flow channels is configured such that each flow channel is perpendicular to the outer edge of the disc-shaped body.
6. The microfluidic device of claim 2 , wherein the flow channels of the plurality of flow channels are arranged in parallel.
7. The microfluidic device of claim 1 , wherein at least one of the fluidic harbors is at an angle other than orthogonal to the flow channel.
8. The microfluidic device of claim 1 , wherein at least one of the fluidic harbors is at an angle orthogonal to the flow channel.
9. The microfluidic device of claim 1 , wherein each of the fluidic harbors are in fluidic communication with the flow channel by a conduit.
10. The microfluidic device of claim 1 , wherein at least one of the fluidic harbors further comprises at least one channel in fluidic communication with the flow channel.
11. The microfluidic device of claim 1 , wherein at least one of the flow channel and the plurality of fluidic harbors comprise a hydrophobic surface.
12. The microfluidic device of claim 1 , wherein at least one of the flow channel and the plurality of fluidic harbors comprise a fluorophilic surface.
13. The microfluidic device of claim 1 , wherein the fluid outlet port is located closer to a center of the disc-shaped body than the fluid inlet port.
14. The microfluidic device of claim 1 , wherein the fluid outlet port is located farther from a center of the disc-shaped body than the fluid inlet port.
15. The microfluidic device of claim 1 , wherein the fluid outlet port is located as close to a center of the disc-shaped device as the fluid inlet port.
16. The microfluidic device of claim 1 , further comprising a plurality of flow cells, wherein each flow cell comprises:
a plurality of flow channels;
a fluid inlet port; and
a fluid outlet port, wherein the fluid outlet port is located closer to the center region of the disc-shaped body than the distal ends of the flow channels.
17. The microfluidic device of claim 16 , wherein each of the flow cells comprises a plurality of the fluid inlet ports, a plurality of the fluid outlet ports, or a combination thereof.
18. The microfluidic device of claim 1 , wherein the disc-shaped body defines an aperture shaped to receive rotational motion from a rotation component of an optical disc drive to rotate the microfluidic device about the central axis of the microfluidic device.
19. The microfluidic device of claim 1 , wherein fluid harbors of the plurality of fluidic harbors are positioned on a bottom of the flow channel.
20. The microfluidic device of claim 1 , wherein each fluidic harbor of the plurality of fluidic harbors defines an opening directly connected to the flow channel, and wherein a first end of each drainage channel of the plurality of drainage channels is directly connected to and in fluidic communication with the flow channel at a position of the flow channel different from the opening.
21. The microfluidic device of claim 20 , wherein a second end of each drainage channel of the plurality of drainage channels is directly connected to a fluidic harbor of the plurality of fluidic harbors at a portion of the fluidic harbor different than the opening.
22. The microfluidic device of claim 20 , wherein each fluidic harbor of the plurality of fluidic harbors is directly connected to two or more drainage channels of the plurality of drainage channels, and wherein each of the two or more drainage channels are directly connected to the flow channel.