Image capture method for a microscope system, and corresponding microscope system
A method ( 200 ) is proposed for furnishing a digital resulting image, using a microscope system ( 1 ) that comprises means (R, L, 41 ) for furnishing microscopic images at different numerical apertures as well as a digital image capture unit ( 50 ). The method encompasses: capturing by means of the digital image capture unit ( 50 ), in the form of digital individual images, at least two microscopic images at different numerical apertures; and comparing respective mutually corresponding image regions of the digital individual images to one another in terms of their image sharpness, the image regions of the digital individual images having the greatest image sharpness being in each case combined to yield the digital resulting image.
1. A method ( 200 ) for furnishing a digital resulting image using a microscope system ( 1 ) that comprises means (R, L, 41 ) for furnishing microscopic images at different numerical apertures as well as a digital image capture unit ( 50 ), wherein the method comprises:
capturing by means of the digital image capture unit ( 50 ), in the form of digital individual images, at least two microscopic images at different numerical apertures, the at least two microscopic images having different depths of field; and
comparing respective mutually corresponding image regions of the digital individual images to one another in terms of their image sharpness, the image regions of the digital individual images having the greatest image sharpness being in each case combined to yield the digital resulting image.
2. The method ( 200 ) according to claim 1 , wherein the comparison of the different image regions of the digital individual images is accomplished in terms of their image sharpness, using at least one value characterizing the image sharpness.
3. The method ( 200 ) according to claim 1 , wherein the mutually corresponding image regions of the digital individual images are defined on the basis of a specified location in the at least two digital individual images.
4. The method ( 200 ) according to claim 3 , wherein the specified location is defined in the form of identical image coordinates and/or identical pixel regions in the at least two digital individual images.
5. The method ( 200 ) according to claim 1 , wherein the mutually corresponding image regions of the digital individual images are ascertained on the basis of an image content detection operation.
6. The method ( 200 ) according to claim 1 , further comprising adjusting at least one aperture diaphragm ( 41 ) in at least one optical channel (L, R) of the microscope system ( 1 ) in order to furnish the at least two microscopic individual images at the different numerical apertures.
7. The method ( 200 ) according to claim 1 , further comprising furnishing and capturing the at least two microscopic individual images at the different numerical apertures simultaneously by means of two optical channels (L, R) each having a fixed numerical aperture.
8. The method ( 200 ) according to claim 1 , wherein the method is repeated for at least two different focus settings of a main objective ( 21 ) of the microscope system ( 1 ) so that one resulting image is obtained for each of the at least two different focus settings.
9. A microscope system ( 1 ) that comprises means (R, L, 41 ) for furnishing microscopic images at different numerical apertures as well as a digital image capture unit ( 50 ), and is configured to carry out a method ( 200 ) as recited in claim 1 .
10. The microscope system ( 1 ) according to claim 9 , wherein the means (L, R, 41 ) for furnishing the microscopic images at the different numerical apertures includes at least one optical channel (L, R) having an aperture diaphragm ( 41 ) having an adjustable diaphragm opening.
11. The microscope system ( 1 ) according to claim 9 , wherein the means (L, R) for furnishing the microscopic images at the different numerical apertures includes two optical channels (L, R) having different and/or adjustable numerical apertures.
12. The microscope system ( 1 ) according to claim 10 , further comprising a control device ( 80 ) in order to apply control to the digital image capture unit ( 50 ) in such a way that the digital image capture unit ( 50 ) captures at least two digital individual images at different diaphragm openings of the aperture diaphragm ( 41 ) in the at least one optical channel (L, R).
13. The microscope system ( 1 ) according to claim 12 , the control device ( 80 ) being embodied to respectively specify a number of digital individual images to be captured and an adjustment magnitude for the aperture diaphragm ( 41 ) in the at least one optical channel (L, R), and to alternatingly carry out a capture of the digital individual images by means of the digital image capture device ( 50 ) and an adjustment of the aperture diaphragm ( 41 ) by an amount equal to the adjustment magnitude.
14. The microscope system ( 1 ) according to claim 9 , wherein the microscope system ( 1 ) is embodied as a stereomicroscope having two stereoscopic channels (L, R), one capture module ( 52 ) of the digital image capture unit ( 50 ) being associated with each of the stereoscopic channels.